Interaction of titanium dioxide nanoparticle with human serum albumin: A spectroscopic approach

Mohd Jahir Khan, Mohamad Yusof Maskat

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: The present study is designed to investigate the interaction of titanium dioxide nanoparticle (TiO2NPs) with human serum albumin (HSA) using spectroscopic techniques. Methods: TiO2NPs was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy. Effect of NPs on the conformation of HSA was evaluated by UV-vis and fluorescence spectroscopies. Results: The characterization result demonstrated that TiO2NPs were somewhat spherical with average diameter of ~32 nm. UV-vis and fluorescence spectroscopic studies showed that NPs form ground state complex with HSA. Conclusion: UV-vis and fluorescence spectroscopy depicted the formation of HSA-TiO2NPs complex induced conformational changes in human serum albumin.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalInternational Journal of Pharmacy and Pharmaceutical Sciences
Volume6
Issue number3
Publication statusPublished - 2014

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Serum Albumin
Nanoparticles
Fluorescence Spectrometry
Fourier Transform Infrared Spectroscopy
Transmission Electron Microscopy
Fluorescence
titanium dioxide

Keywords

  • Human serum albumin
  • Protein conformation
  • Protein-nanoparticle interaction
  • Titanium dioxide nanoparticle

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

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abstract = "Objective: The present study is designed to investigate the interaction of titanium dioxide nanoparticle (TiO2NPs) with human serum albumin (HSA) using spectroscopic techniques. Methods: TiO2NPs was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy. Effect of NPs on the conformation of HSA was evaluated by UV-vis and fluorescence spectroscopies. Results: The characterization result demonstrated that TiO2NPs were somewhat spherical with average diameter of ~32 nm. UV-vis and fluorescence spectroscopic studies showed that NPs form ground state complex with HSA. Conclusion: UV-vis and fluorescence spectroscopy depicted the formation of HSA-TiO2NPs complex induced conformational changes in human serum albumin.",
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T1 - Interaction of titanium dioxide nanoparticle with human serum albumin

T2 - A spectroscopic approach

AU - Khan, Mohd Jahir

AU - Maskat, Mohamad Yusof

PY - 2014

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N2 - Objective: The present study is designed to investigate the interaction of titanium dioxide nanoparticle (TiO2NPs) with human serum albumin (HSA) using spectroscopic techniques. Methods: TiO2NPs was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy. Effect of NPs on the conformation of HSA was evaluated by UV-vis and fluorescence spectroscopies. Results: The characterization result demonstrated that TiO2NPs were somewhat spherical with average diameter of ~32 nm. UV-vis and fluorescence spectroscopic studies showed that NPs form ground state complex with HSA. Conclusion: UV-vis and fluorescence spectroscopy depicted the formation of HSA-TiO2NPs complex induced conformational changes in human serum albumin.

AB - Objective: The present study is designed to investigate the interaction of titanium dioxide nanoparticle (TiO2NPs) with human serum albumin (HSA) using spectroscopic techniques. Methods: TiO2NPs was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy. Effect of NPs on the conformation of HSA was evaluated by UV-vis and fluorescence spectroscopies. Results: The characterization result demonstrated that TiO2NPs were somewhat spherical with average diameter of ~32 nm. UV-vis and fluorescence spectroscopic studies showed that NPs form ground state complex with HSA. Conclusion: UV-vis and fluorescence spectroscopy depicted the formation of HSA-TiO2NPs complex induced conformational changes in human serum albumin.

KW - Human serum albumin

KW - Protein conformation

KW - Protein-nanoparticle interaction

KW - Titanium dioxide nanoparticle

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