Interaksi antara kompleks N, N’-bis [4-[1-(2-hidroksietoksi)] saliksalidin]-fenildiamina-nikel(II) dan kuprum(II) bersama DNA G-kuadrupleks)

Translated title of the contribution: Interaction of n,n’-bis[4-[1-(2-hydroxyethoxy)] salicylidene]-phenyldiamine-nickel(II) and copper(II) complexes with G-quadruplex DNA

Shamsuddin Roshatiara, Muhammad Ameerullah Sahudin, Nur Hasyareeda Hassan, Nurul Huda Abd Karim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Binding studies of metal complexes with DNA has attracted many interests due to their importance in cancer therapy and molecular biology. In this study, N,N’-bis[4-[1-(2-hydroxyethoxy)]salicylidene]-phenyldiamine and its metal Ni(II) and Cu(II) complexes have been synthesized and characterized. The complexes were structurally characterized using standard spectroscopic 1D1H, 2D COSY, HMQC and13C NMR spectroscopy, FTIR spectroscopy and ESI-MS spectrometry. The binding interaction between synthesized metal complexes and G-quadruplex DNA has been investigated using UV/Vis DNA titration study. Ni(II) and Cu(II) complexes bind strongly to G-quadruplex DNA (Kb = (5.42 ± 2.48) × 106M-1 and (1.42 ± 2.48) × 106M-1 respectively) via end stacking mode. Both complexes show selectivity for G-quadruplex DNA over CT-DNA by 2.15-and 1.82-fold, respectively.

Original languageMalay
Pages (from-to)544-551
Number of pages8
JournalMalaysian Journal of Analytical Sciences
Volume21
Issue number3
DOIs
Publication statusPublished - 2017

Fingerprint

Nickel
Copper
DNA
Coordination Complexes
Molecular biology
Titration
Spectrometry
Nuclear magnetic resonance spectroscopy
Metals
Spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Interaksi antara kompleks N, N’-bis [4-[1-(2-hidroksietoksi)] saliksalidin]-fenildiamina-nikel(II) dan kuprum(II) bersama DNA G-kuadrupleks)",
abstract = "Binding studies of metal complexes with DNA has attracted many interests due to their importance in cancer therapy and molecular biology. In this study, N,N’-bis[4-[1-(2-hydroxyethoxy)]salicylidene]-phenyldiamine and its metal Ni(II) and Cu(II) complexes have been synthesized and characterized. The complexes were structurally characterized using standard spectroscopic 1D1H, 2D COSY, HMQC and13C NMR spectroscopy, FTIR spectroscopy and ESI-MS spectrometry. The binding interaction between synthesized metal complexes and G-quadruplex DNA has been investigated using UV/Vis DNA titration study. Ni(II) and Cu(II) complexes bind strongly to G-quadruplex DNA (Kb = (5.42 ± 2.48) × 106M-1 and (1.42 ± 2.48) × 106M-1 respectively) via end stacking mode. Both complexes show selectivity for G-quadruplex DNA over CT-DNA by 2.15-and 1.82-fold, respectively.",
keywords = "DNA binding, G-quadruplex DNA, Metal complexes",
author = "Shamsuddin Roshatiara and Sahudin, {Muhammad Ameerullah} and Hassan, {Nur Hasyareeda} and {Abd Karim}, {Nurul Huda}",
year = "2017",
doi = "10.17576/mjas-2017-2103-04",
language = "Malay",
volume = "21",
pages = "544--551",
journal = "Malaysian Journal of Analytical Sciences",
issn = "1394-2506",
publisher = "Faculty of Science and Technology, Universiti Kebangsaan Malaysia",
number = "3",

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TY - JOUR

T1 - Interaksi antara kompleks N, N’-bis [4-[1-(2-hidroksietoksi)] saliksalidin]-fenildiamina-nikel(II) dan kuprum(II) bersama DNA G-kuadrupleks)

AU - Roshatiara, Shamsuddin

AU - Sahudin, Muhammad Ameerullah

AU - Hassan, Nur Hasyareeda

AU - Abd Karim, Nurul Huda

PY - 2017

Y1 - 2017

N2 - Binding studies of metal complexes with DNA has attracted many interests due to their importance in cancer therapy and molecular biology. In this study, N,N’-bis[4-[1-(2-hydroxyethoxy)]salicylidene]-phenyldiamine and its metal Ni(II) and Cu(II) complexes have been synthesized and characterized. The complexes were structurally characterized using standard spectroscopic 1D1H, 2D COSY, HMQC and13C NMR spectroscopy, FTIR spectroscopy and ESI-MS spectrometry. The binding interaction between synthesized metal complexes and G-quadruplex DNA has been investigated using UV/Vis DNA titration study. Ni(II) and Cu(II) complexes bind strongly to G-quadruplex DNA (Kb = (5.42 ± 2.48) × 106M-1 and (1.42 ± 2.48) × 106M-1 respectively) via end stacking mode. Both complexes show selectivity for G-quadruplex DNA over CT-DNA by 2.15-and 1.82-fold, respectively.

AB - Binding studies of metal complexes with DNA has attracted many interests due to their importance in cancer therapy and molecular biology. In this study, N,N’-bis[4-[1-(2-hydroxyethoxy)]salicylidene]-phenyldiamine and its metal Ni(II) and Cu(II) complexes have been synthesized and characterized. The complexes were structurally characterized using standard spectroscopic 1D1H, 2D COSY, HMQC and13C NMR spectroscopy, FTIR spectroscopy and ESI-MS spectrometry. The binding interaction between synthesized metal complexes and G-quadruplex DNA has been investigated using UV/Vis DNA titration study. Ni(II) and Cu(II) complexes bind strongly to G-quadruplex DNA (Kb = (5.42 ± 2.48) × 106M-1 and (1.42 ± 2.48) × 106M-1 respectively) via end stacking mode. Both complexes show selectivity for G-quadruplex DNA over CT-DNA by 2.15-and 1.82-fold, respectively.

KW - DNA binding

KW - G-quadruplex DNA

KW - Metal complexes

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