Interaksi Asid Hyaluronik (HA) dan Dipalmitoilfosfatidilkolina (DPPC) dan Kesan Penyinaran Gama ke atas Kestabilan HA-Lipid

Translated title of the contribution: Interaction of hyaluronic acid (Ha) with dipalmitoylphosphatidylcholine (dppc) and its effect on the stability of ha-lipid to gamma irradiation

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DPPC lipids are extensively utilized as a biomembrane model as they resembled biological cells and their significance in various physiological functions particularly in drug delivery system. The synovial joint fluid containing hyaluronic acid, proteins, proteoglycans and lipids. Hyaluronic acid (HA) is the most signified constituent in the synovial joint fluid and functions as lubricant, nutrient carrier and shock absorber. Gamma irradiation has also been discovered to be effeciently in depolymerizing and cleaving molecular chains which initiated changes in chemical composition as well as its physiological functions due to free radical. This research are conducted to investigate the hyaluronic acid (HA) with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) interaction in form of vesicles and its effect on the stability of HA-DPPC structure to gamma radiation. The size distribution of DPPC vesicles measured by Dynamic Light Scattering (DLS) is between 100 to 200 nm in diameter. HA was added into the vesicles and characterized by using TEM to determine vesicle size distributions, fusion and rupture of HA-DPPC structure. The results demonstrated that the size of the vesicles approximately between 200 to 400 nm which caused by vesicles fusion with HA and formed even larger vesicles. After being irradiated by 0 to 200 Gy, the Z-average of DPPC vesicles decreased to 164.7 nm meanwhile for DPPC in presence of HA, the Z-average is 391.6 nm. FTIR spectra were carried out to clarified formation of double bonds at ~1700-1750 cm-1 which leads to formation of pyrancarboxylic acid rings and modified the structure of HA, hence its effect the structure of the DPPC vesicles.

Original languageUndefined/Unknown
Pages (from-to)173-178
Number of pages6
JournalMalaysian Journal of Analytical Sciences
Volume19
Issue number1
Publication statusPublished - 2015

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Hyaluronic Acid
Phosphatidylcholines
Irradiation
Lipids
Fusion reactions
Shock absorbers
Fluids
Dynamic light scattering
Proteoglycans
Gamma rays
Nutrients
Free Radicals
Lubricants
Transmission electron microscopy
Acids

Keywords

  • Dipalmitoylphosphatidylcholine (DPPC)
  • Gamma irradiation
  • Hyaluronic acid (HA)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

@article{63b1a574d445427b9ba1c542d2b5cf83,
title = "Interaksi Asid Hyaluronik (HA) dan Dipalmitoilfosfatidilkolina (DPPC) dan Kesan Penyinaran Gama ke atas Kestabilan HA-Lipid",
abstract = "DPPC lipids are extensively utilized as a biomembrane model as they resembled biological cells and their significance in various physiological functions particularly in drug delivery system. The synovial joint fluid containing hyaluronic acid, proteins, proteoglycans and lipids. Hyaluronic acid (HA) is the most signified constituent in the synovial joint fluid and functions as lubricant, nutrient carrier and shock absorber. Gamma irradiation has also been discovered to be effeciently in depolymerizing and cleaving molecular chains which initiated changes in chemical composition as well as its physiological functions due to free radical. This research are conducted to investigate the hyaluronic acid (HA) with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) interaction in form of vesicles and its effect on the stability of HA-DPPC structure to gamma radiation. The size distribution of DPPC vesicles measured by Dynamic Light Scattering (DLS) is between 100 to 200 nm in diameter. HA was added into the vesicles and characterized by using TEM to determine vesicle size distributions, fusion and rupture of HA-DPPC structure. The results demonstrated that the size of the vesicles approximately between 200 to 400 nm which caused by vesicles fusion with HA and formed even larger vesicles. After being irradiated by 0 to 200 Gy, the Z-average of DPPC vesicles decreased to 164.7 nm meanwhile for DPPC in presence of HA, the Z-average is 391.6 nm. FTIR spectra were carried out to clarified formation of double bonds at ~1700-1750 cm-1 which leads to formation of pyrancarboxylic acid rings and modified the structure of HA, hence its effect the structure of the DPPC vesicles.",
keywords = "Dipalmitoylphosphatidylcholine (DPPC), Gamma irradiation, Hyaluronic acid (HA)",
author = "Ahmad, {Ainee Fatimah} and {Abdul Rahman}, Irman and Mohd, {Hur Munawar Kabir} and {K.P. Kunchi Mohamed}, Faizal and Shahidan Radiman and Yasir, {Muhamad Samudi}",
year = "2015",
language = "Undefined/Unknown",
volume = "19",
pages = "173--178",
journal = "Malaysian Journal of Analytical Sciences",
issn = "1394-2506",
publisher = "Faculty of Science and Technology, Universiti Kebangsaan Malaysia",
number = "1",

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TY - JOUR

T1 - Interaksi Asid Hyaluronik (HA) dan Dipalmitoilfosfatidilkolina (DPPC) dan Kesan Penyinaran Gama ke atas Kestabilan HA-Lipid

AU - Ahmad, Ainee Fatimah

AU - Abdul Rahman, Irman

AU - Mohd, Hur Munawar Kabir

AU - K.P. Kunchi Mohamed, Faizal

AU - Radiman, Shahidan

AU - Yasir, Muhamad Samudi

PY - 2015

Y1 - 2015

N2 - DPPC lipids are extensively utilized as a biomembrane model as they resembled biological cells and their significance in various physiological functions particularly in drug delivery system. The synovial joint fluid containing hyaluronic acid, proteins, proteoglycans and lipids. Hyaluronic acid (HA) is the most signified constituent in the synovial joint fluid and functions as lubricant, nutrient carrier and shock absorber. Gamma irradiation has also been discovered to be effeciently in depolymerizing and cleaving molecular chains which initiated changes in chemical composition as well as its physiological functions due to free radical. This research are conducted to investigate the hyaluronic acid (HA) with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) interaction in form of vesicles and its effect on the stability of HA-DPPC structure to gamma radiation. The size distribution of DPPC vesicles measured by Dynamic Light Scattering (DLS) is between 100 to 200 nm in diameter. HA was added into the vesicles and characterized by using TEM to determine vesicle size distributions, fusion and rupture of HA-DPPC structure. The results demonstrated that the size of the vesicles approximately between 200 to 400 nm which caused by vesicles fusion with HA and formed even larger vesicles. After being irradiated by 0 to 200 Gy, the Z-average of DPPC vesicles decreased to 164.7 nm meanwhile for DPPC in presence of HA, the Z-average is 391.6 nm. FTIR spectra were carried out to clarified formation of double bonds at ~1700-1750 cm-1 which leads to formation of pyrancarboxylic acid rings and modified the structure of HA, hence its effect the structure of the DPPC vesicles.

AB - DPPC lipids are extensively utilized as a biomembrane model as they resembled biological cells and their significance in various physiological functions particularly in drug delivery system. The synovial joint fluid containing hyaluronic acid, proteins, proteoglycans and lipids. Hyaluronic acid (HA) is the most signified constituent in the synovial joint fluid and functions as lubricant, nutrient carrier and shock absorber. Gamma irradiation has also been discovered to be effeciently in depolymerizing and cleaving molecular chains which initiated changes in chemical composition as well as its physiological functions due to free radical. This research are conducted to investigate the hyaluronic acid (HA) with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) interaction in form of vesicles and its effect on the stability of HA-DPPC structure to gamma radiation. The size distribution of DPPC vesicles measured by Dynamic Light Scattering (DLS) is between 100 to 200 nm in diameter. HA was added into the vesicles and characterized by using TEM to determine vesicle size distributions, fusion and rupture of HA-DPPC structure. The results demonstrated that the size of the vesicles approximately between 200 to 400 nm which caused by vesicles fusion with HA and formed even larger vesicles. After being irradiated by 0 to 200 Gy, the Z-average of DPPC vesicles decreased to 164.7 nm meanwhile for DPPC in presence of HA, the Z-average is 391.6 nm. FTIR spectra were carried out to clarified formation of double bonds at ~1700-1750 cm-1 which leads to formation of pyrancarboxylic acid rings and modified the structure of HA, hence its effect the structure of the DPPC vesicles.

KW - Dipalmitoylphosphatidylcholine (DPPC)

KW - Gamma irradiation

KW - Hyaluronic acid (HA)

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M3 - Article

VL - 19

SP - 173

EP - 178

JO - Malaysian Journal of Analytical Sciences

JF - Malaysian Journal of Analytical Sciences

SN - 1394-2506

IS - 1

ER -