Insulin-Transferrin-Selenium prevent human chondrocyte dedifferentiation and promote the formation of high quality tissue engineered human hyaline cartilage

Chua Kien Hui, B. S. Aminuddin, N. H. Fuzina, Ruszymah Idrus

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

This study was to investigate the effects of Insulin-Transferrin-Selenium (ITS) on the proliferation and quantitative gene expression of adult human nasal septum chondrocytes in monolayer culture expansion and the formation of tissue engineered hyaline cartilage. Effects of ITS on human nasal septum chondrocytes monolayer culture expansion and gene expression were evaluated in various culture media either added with 2% fetal bovine serum (FBS) or 1ng/mL basic fibroblast growth factor plus 1ng/mL transforming growth factor or both serum and growth factors supplementation in comparison with medium added with 10%FBS. Chondrocytes cultured in medium added with 2% fetal bovine serum and growth factors either supplemented with or without ITS were then mixed with pluronic F-127 hydrogel for in vivo tissue engineered cartilage formation in nude mice model. Engineered tissues were removed after 8 weeks of implantation and evaluated with histological staining, immunohistochemistry, transmission electron microscopy and quantitative gene expression analysis. ITS promoted human chondrocytes proliferation and reduced chondrocytes dedifferentiation in media supplemented with serum and growth factors. ITS with 2% FBS and growth factors provided 15-fold increased in chondrocytes number by the end of the culture period compared to the standard culture medium used in chondrocytes culture (medium added with 10% FBS). Engineered tissue resulted from ITS supplementation demonstrated higher quality of cartilage formation. In conclusion, our study has demonstrated the benefits of ITS supplementation in human chondrocytes monolayer culture and tissue engineering cartilage formation.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalEuropean Cells and Materials
Volume9
Publication statusPublished - Jan 2005

Fingerprint

Hyaline Cartilage
Insulin
Cartilage
Selenium
Transferrin
Chondrocytes
Tissue
Intercellular Signaling Peptides and Proteins
Serum
Gene expression
Culture Media
Monolayers
Nasal Septum
Gene Expression
Poloxamer
Tissue Expansion
Hydrogel
Transforming Growth Factors
Fibroblast Growth Factor 2
Fibroblasts

Keywords

  • Basic fibroblast growth factor
  • Human nasal septum chondrocytes
  • Hyaline cartilage
  • Hydrogel
  • Insulin-Transferrin-Selenium
  • Monolayer expansion
  • Quantitative gene expression
  • Tissue engineering
  • Transforming growth factor

ASJC Scopus subject areas

  • Biochemistry
  • Biomaterials

Cite this

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title = "Insulin-Transferrin-Selenium prevent human chondrocyte dedifferentiation and promote the formation of high quality tissue engineered human hyaline cartilage",
abstract = "This study was to investigate the effects of Insulin-Transferrin-Selenium (ITS) on the proliferation and quantitative gene expression of adult human nasal septum chondrocytes in monolayer culture expansion and the formation of tissue engineered hyaline cartilage. Effects of ITS on human nasal septum chondrocytes monolayer culture expansion and gene expression were evaluated in various culture media either added with 2{\%} fetal bovine serum (FBS) or 1ng/mL basic fibroblast growth factor plus 1ng/mL transforming growth factor or both serum and growth factors supplementation in comparison with medium added with 10{\%}FBS. Chondrocytes cultured in medium added with 2{\%} fetal bovine serum and growth factors either supplemented with or without ITS were then mixed with pluronic F-127 hydrogel for in vivo tissue engineered cartilage formation in nude mice model. Engineered tissues were removed after 8 weeks of implantation and evaluated with histological staining, immunohistochemistry, transmission electron microscopy and quantitative gene expression analysis. ITS promoted human chondrocytes proliferation and reduced chondrocytes dedifferentiation in media supplemented with serum and growth factors. ITS with 2{\%} FBS and growth factors provided 15-fold increased in chondrocytes number by the end of the culture period compared to the standard culture medium used in chondrocytes culture (medium added with 10{\%} FBS). Engineered tissue resulted from ITS supplementation demonstrated higher quality of cartilage formation. In conclusion, our study has demonstrated the benefits of ITS supplementation in human chondrocytes monolayer culture and tissue engineering cartilage formation.",
keywords = "Basic fibroblast growth factor, Human nasal septum chondrocytes, Hyaline cartilage, Hydrogel, Insulin-Transferrin-Selenium, Monolayer expansion, Quantitative gene expression, Tissue engineering, Transforming growth factor",
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AU - Idrus, Ruszymah

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