Insecticide resistance status and mechanisms in Malaysian Blattella germanica (Linnaeus)

H. L. Lee, W. D. Tien, Baharudin Omar

Research output: Contribution to journalArticle

Abstract

The insecticide resistance status of 4 strains of adult male Blattella germanica, viz M (Malacca), E (England), F (restaurant) and K (cafeteria) against malathion and bendiocarb compared with a reference susceptible strain (S) was determined by using a modified WHO bioassay method. The results indicated that all the 4 strains were resistant to the insecticides albeit in different degrees. Resistance ratios for malathion ranged from 1.85-41.07-fold, whereas that of bendiocarb ranged from 1.68-4.83-fold. The biochemical microplate enzyme assays technique employed indicated that the resistance in M and E strains were attributed to acetylcholinesterase insensitivity. Multiple resistance was not detected in any of the 4 strains. Parameters of the identified resistance mechanism correlated well with the observed level of resistance. Agar gel electrophoresis showed that variations in esterase isoenzymes did not confer organophosphate and carbamate resistance to the 4 strains.

Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume28
Issue number1
Publication statusPublished - Mar 1997
Externally publishedYes

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Insecticide Resistance
Malathion
Restaurants
Carbamates
Organophosphates
Agar Gel Electrophoresis
Enzyme Assays
Esterases
Acetylcholinesterase
Insecticides
Biological Assay
England
Isoenzymes
bendiocarb

ASJC Scopus subject areas

  • Medicine(all)

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Insecticide resistance status and mechanisms in Malaysian Blattella germanica (Linnaeus). / Lee, H. L.; Tien, W. D.; Omar, Baharudin.

In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 28, No. 1, 03.1997, p. 212-217.

Research output: Contribution to journalArticle

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