Pembangunan Inokulum untuk Penghasilan Lipid dan Asid Gamma Linolenik (GLA) oleh Cunninghamella bainieri 2A1

Translated title of the contribution: Inoculum development for lipid and gamma linoenic acids production by cunninghamella bainieri 2A1

Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Inoculum for a local isolate of oleaginus fungi, Cunninghamella bainieri 2A1 was developed by establishing the inoculum types, age and size for growth, lipid and gamma linolenic acid (GLA) production. Cultivation was carried out in 500 mL shake flask containing 200 mL of nitrogen limiting medium at 30°C and 250 rpm agitation. Direct inoculation of spores into the production cultures gave higher productivity of lipid production 0.71 (g/L/day) than using 24 and 48 vegetative cells cultures which gave only 0.51 and 0.45 (g/L/day), respectively. Besides, production of GLA (5.3 × 10 -2 g/g lipid less biomass) in the cultures which directly inoculated with spores (1 × 10 5 spores/mL) was 23% higher than those produced in the cultures started with vegetative cells. Spore concentration of 1 × 10 3 spores/mL produced pelletal growth in size of 1.04 mm which contain 40% (g/g biomass) lipid and 8.34 × 10 -2 (g/g lipid less biomass).

Original languageUndefined/Unknown
Pages (from-to)431-437
Number of pages7
JournalSains Malaysiana
Volume41
Issue number4
Publication statusPublished - Apr 2012

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Cunninghamella
inoculum
spores
acids
lipids
gamma-linolenic acid
vegetative cells
biomass
agitation
cell culture
fungi
nitrogen

Keywords

  • Cunninghamella bainieri
  • Gamma linolenic acid
  • Inoculum
  • Lipid
  • Pellet

ASJC Scopus subject areas

  • General

Cite this

Pembangunan Inokulum untuk Penghasilan Lipid dan Asid Gamma Linolenik (GLA) oleh Cunninghamella bainieri 2A1. / Muhid, Farhila; Kader, Abdul Jalil Abdul; Wan Yusoff, Wan Mohtar; Omar, Othman; Abdul Hamid, Aidil.

In: Sains Malaysiana, Vol. 41, No. 4, 04.2012, p. 431-437.

Research output: Contribution to journalArticle

Muhid, Farhila ; Kader, Abdul Jalil Abdul ; Wan Yusoff, Wan Mohtar ; Omar, Othman ; Abdul Hamid, Aidil. / Pembangunan Inokulum untuk Penghasilan Lipid dan Asid Gamma Linolenik (GLA) oleh Cunninghamella bainieri 2A1. In: Sains Malaysiana. 2012 ; Vol. 41, No. 4. pp. 431-437.
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