Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice

Menaga Ilangkovan, Ibrahim Jantan, Osman Mesaik Mohammed Ahmed Hassan, Bukhari Syed Nasir Abbas

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Phyllanthus amarus has been shown to have strong inhibitory effects on phagocytic activity of human neutrophils and on cellular immune responses in Wistar–Kyoto rats. In this study, we investigated the effects of daily treatment of standardized extract of P. amarus at 50, 100 and 200 mg/kg for 14 days in Balb/C mice by measuring the myeloperoxidase activity (MPO), nitric oxide (NO) release, macrophage phagocytosis, swelling of footpad in delayed type hypersensitivity (DTH), and serum immunoglobulins, ceruloplasmin and lysozyme levels. Qualitative and quantitative analyses of the extract using validated reversed-phase HPLC methods identified phyllanthin, hypophyllanthin, corilagin and geraniin as the biomarkers. Significant dose-dependent inhibitions of MPO activity and NO release were observed in treated mice. The extract also inhibited E. coli phagocytic capacity of peritoneal macrophages of treated mice and inhibited the sheep red blood cells (sRBC)-induced swelling rate of mice paw in the DTH. There was also a significant decrease in non-specific humoral immunity including ceruloplasmin and lysozyme levels in the extract-fed groups as well as the release of serum level immunoglobulins. The strong inhibitory effects of the extract on the cellular and humoral immune responses suggest the potential of the plant to be developed as an effective immunosuppressive agent.

Original languageEnglish
Pages (from-to)1330-1338
Number of pages9
JournalPhytotherapy Research
DOIs
Publication statusPublished - 1 Aug 2016

Fingerprint

Phyllanthus
Humoral Immunity
Cellular Immunity
Ceruloplasmin
Delayed Hypersensitivity
Muramidase
Peroxidase
Immunoglobulins
Nitric Oxide
Peritoneal Macrophages
Immunosuppressive Agents
Serum
Phagocytosis
Innate Immunity
Human Activities
Sheep
Neutrophils
Erythrocytes
Biomarkers
Macrophages

Keywords

  • cellular immunity
  • humoral immunity
  • immunoglobulins
  • immunosuppressant
  • Phyllanthus amarus
  • standardized extract

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology

Cite this

Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice. / Ilangkovan, Menaga; Jantan, Ibrahim; Mohammed Ahmed Hassan, Osman Mesaik; Syed Nasir Abbas, Bukhari.

In: Phytotherapy Research, 01.08.2016, p. 1330-1338.

Research output: Contribution to journalArticle

Ilangkovan, Menaga ; Jantan, Ibrahim ; Mohammed Ahmed Hassan, Osman Mesaik ; Syed Nasir Abbas, Bukhari. / Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice. In: Phytotherapy Research. 2016 ; pp. 1330-1338.
@article{9b1924d315c149ccbd1544805a181e34,
title = "Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice",
abstract = "Phyllanthus amarus has been shown to have strong inhibitory effects on phagocytic activity of human neutrophils and on cellular immune responses in Wistar–Kyoto rats. In this study, we investigated the effects of daily treatment of standardized extract of P. amarus at 50, 100 and 200 mg/kg for 14 days in Balb/C mice by measuring the myeloperoxidase activity (MPO), nitric oxide (NO) release, macrophage phagocytosis, swelling of footpad in delayed type hypersensitivity (DTH), and serum immunoglobulins, ceruloplasmin and lysozyme levels. Qualitative and quantitative analyses of the extract using validated reversed-phase HPLC methods identified phyllanthin, hypophyllanthin, corilagin and geraniin as the biomarkers. Significant dose-dependent inhibitions of MPO activity and NO release were observed in treated mice. The extract also inhibited E. coli phagocytic capacity of peritoneal macrophages of treated mice and inhibited the sheep red blood cells (sRBC)-induced swelling rate of mice paw in the DTH. There was also a significant decrease in non-specific humoral immunity including ceruloplasmin and lysozyme levels in the extract-fed groups as well as the release of serum level immunoglobulins. The strong inhibitory effects of the extract on the cellular and humoral immune responses suggest the potential of the plant to be developed as an effective immunosuppressive agent.",
keywords = "cellular immunity, humoral immunity, immunoglobulins, immunosuppressant, Phyllanthus amarus, standardized extract",
author = "Menaga Ilangkovan and Ibrahim Jantan and {Mohammed Ahmed Hassan}, {Osman Mesaik} and {Syed Nasir Abbas}, Bukhari",
year = "2016",
month = "8",
day = "1",
doi = "10.1002/ptr.5633",
language = "English",
pages = "1330--1338",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Inhibitory Effects of the Standardized Extract of Phyllanthus amarus on Cellular and Humoral Immune Responses in Balb/C Mice

AU - Ilangkovan, Menaga

AU - Jantan, Ibrahim

AU - Mohammed Ahmed Hassan, Osman Mesaik

AU - Syed Nasir Abbas, Bukhari

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Phyllanthus amarus has been shown to have strong inhibitory effects on phagocytic activity of human neutrophils and on cellular immune responses in Wistar–Kyoto rats. In this study, we investigated the effects of daily treatment of standardized extract of P. amarus at 50, 100 and 200 mg/kg for 14 days in Balb/C mice by measuring the myeloperoxidase activity (MPO), nitric oxide (NO) release, macrophage phagocytosis, swelling of footpad in delayed type hypersensitivity (DTH), and serum immunoglobulins, ceruloplasmin and lysozyme levels. Qualitative and quantitative analyses of the extract using validated reversed-phase HPLC methods identified phyllanthin, hypophyllanthin, corilagin and geraniin as the biomarkers. Significant dose-dependent inhibitions of MPO activity and NO release were observed in treated mice. The extract also inhibited E. coli phagocytic capacity of peritoneal macrophages of treated mice and inhibited the sheep red blood cells (sRBC)-induced swelling rate of mice paw in the DTH. There was also a significant decrease in non-specific humoral immunity including ceruloplasmin and lysozyme levels in the extract-fed groups as well as the release of serum level immunoglobulins. The strong inhibitory effects of the extract on the cellular and humoral immune responses suggest the potential of the plant to be developed as an effective immunosuppressive agent.

AB - Phyllanthus amarus has been shown to have strong inhibitory effects on phagocytic activity of human neutrophils and on cellular immune responses in Wistar–Kyoto rats. In this study, we investigated the effects of daily treatment of standardized extract of P. amarus at 50, 100 and 200 mg/kg for 14 days in Balb/C mice by measuring the myeloperoxidase activity (MPO), nitric oxide (NO) release, macrophage phagocytosis, swelling of footpad in delayed type hypersensitivity (DTH), and serum immunoglobulins, ceruloplasmin and lysozyme levels. Qualitative and quantitative analyses of the extract using validated reversed-phase HPLC methods identified phyllanthin, hypophyllanthin, corilagin and geraniin as the biomarkers. Significant dose-dependent inhibitions of MPO activity and NO release were observed in treated mice. The extract also inhibited E. coli phagocytic capacity of peritoneal macrophages of treated mice and inhibited the sheep red blood cells (sRBC)-induced swelling rate of mice paw in the DTH. There was also a significant decrease in non-specific humoral immunity including ceruloplasmin and lysozyme levels in the extract-fed groups as well as the release of serum level immunoglobulins. The strong inhibitory effects of the extract on the cellular and humoral immune responses suggest the potential of the plant to be developed as an effective immunosuppressive agent.

KW - cellular immunity

KW - humoral immunity

KW - immunoglobulins

KW - immunosuppressant

KW - Phyllanthus amarus

KW - standardized extract

UR - http://www.scopus.com/inward/record.url?scp=84982854403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982854403&partnerID=8YFLogxK

U2 - 10.1002/ptr.5633

DO - 10.1002/ptr.5633

M3 - Article

SP - 1330

EP - 1338

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

ER -