Inhibitory effects of hylligenin and quebrachitol isolated from mitrephora vulpina on platelet activating factor receptor binding and platelet aggregation

Bushra Abdulkarim Moharam, Ibrahim Jantan, Juriyati Jalil, Khozirah Shaari

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids-oxoputerine and liriodenine-were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand and their antiplatelet aggregation effect in human whole blood induced by arachidonic acid (AA), collagen and adenosine diphosphate (ADP). Of all the compounds tested, phylligenin and quebrachitol exhibited potent and concentration-dependent inhibitory effects on PAF receptor binding, with IC50 values of 13.1 and 42.2 μM, respectively. The IC 50 value of phylligenin was comparable to that of cedrol (10.2 μM), a potent PAF antagonist. Phylligenin also showed strong dose-dependent inhibitory activity on platelet aggregation induced by AA and ADP.

Original languageEnglish
Pages (from-to)7840-7848
Number of pages9
JournalMolecules
Volume15
Issue number11
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Receptor Aggregation
Platelets
platelets
Platelet Aggregation
Agglomeration
Platelet Activating Factor
Arachidonic Acid
Adenosine Diphosphate
Stigmasterol
adenosine diphosphate
Alkaloids
Inhibitory Concentration 50
Blood
Collagen
Blood Platelets
Rabbits
Ligands
diphosphates
acids
alkaloids

Keywords

  • Antiplatelet activity
  • Mitrephora vulpina
  • Phylligenin
  • Platelet activating factor (PAF)antagonist
  • Quebrachitol

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Inhibitory effects of hylligenin and quebrachitol isolated from mitrephora vulpina on platelet activating factor receptor binding and platelet aggregation. / Moharam, Bushra Abdulkarim; Jantan, Ibrahim; Jalil, Juriyati; Shaari, Khozirah.

In: Molecules, Vol. 15, No. 11, 11.2010, p. 7840-7848.

Research output: Contribution to journalArticle

@article{8fe0882856c147bfa4857b1e21f2e741,
title = "Inhibitory effects of hylligenin and quebrachitol isolated from mitrephora vulpina on platelet activating factor receptor binding and platelet aggregation",
abstract = "Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids-oxoputerine and liriodenine-were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand and their antiplatelet aggregation effect in human whole blood induced by arachidonic acid (AA), collagen and adenosine diphosphate (ADP). Of all the compounds tested, phylligenin and quebrachitol exhibited potent and concentration-dependent inhibitory effects on PAF receptor binding, with IC50 values of 13.1 and 42.2 μM, respectively. The IC 50 value of phylligenin was comparable to that of cedrol (10.2 μM), a potent PAF antagonist. Phylligenin also showed strong dose-dependent inhibitory activity on platelet aggregation induced by AA and ADP.",
keywords = "Antiplatelet activity, Mitrephora vulpina, Phylligenin, Platelet activating factor (PAF)antagonist, Quebrachitol",
author = "Moharam, {Bushra Abdulkarim} and Ibrahim Jantan and Juriyati Jalil and Khozirah Shaari",
year = "2010",
month = "11",
doi = "10.3390/molecules15117840",
language = "English",
volume = "15",
pages = "7840--7848",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "11",

}

TY - JOUR

T1 - Inhibitory effects of hylligenin and quebrachitol isolated from mitrephora vulpina on platelet activating factor receptor binding and platelet aggregation

AU - Moharam, Bushra Abdulkarim

AU - Jantan, Ibrahim

AU - Jalil, Juriyati

AU - Shaari, Khozirah

PY - 2010/11

Y1 - 2010/11

N2 - Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids-oxoputerine and liriodenine-were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand and their antiplatelet aggregation effect in human whole blood induced by arachidonic acid (AA), collagen and adenosine diphosphate (ADP). Of all the compounds tested, phylligenin and quebrachitol exhibited potent and concentration-dependent inhibitory effects on PAF receptor binding, with IC50 values of 13.1 and 42.2 μM, respectively. The IC 50 value of phylligenin was comparable to that of cedrol (10.2 μM), a potent PAF antagonist. Phylligenin also showed strong dose-dependent inhibitory activity on platelet aggregation induced by AA and ADP.

AB - Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids-oxoputerine and liriodenine-were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand and their antiplatelet aggregation effect in human whole blood induced by arachidonic acid (AA), collagen and adenosine diphosphate (ADP). Of all the compounds tested, phylligenin and quebrachitol exhibited potent and concentration-dependent inhibitory effects on PAF receptor binding, with IC50 values of 13.1 and 42.2 μM, respectively. The IC 50 value of phylligenin was comparable to that of cedrol (10.2 μM), a potent PAF antagonist. Phylligenin also showed strong dose-dependent inhibitory activity on platelet aggregation induced by AA and ADP.

KW - Antiplatelet activity

KW - Mitrephora vulpina

KW - Phylligenin

KW - Platelet activating factor (PAF)antagonist

KW - Quebrachitol

UR - http://www.scopus.com/inward/record.url?scp=78649492678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649492678&partnerID=8YFLogxK

U2 - 10.3390/molecules15117840

DO - 10.3390/molecules15117840

M3 - Article

C2 - 21060292

AN - SCOPUS:78649492678

VL - 15

SP - 7840

EP - 7848

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 11

ER -