Inhibitory effect of triterpenoids from Dillenia serrata (Dilleniaceae) on prostaglandin e2 production and quantitative HPLC analysis of its koetjapic acid and betulinic acid contents

Juriyati Jalil, Carla W. Sabandar, Norizan Ahmat, Jamia Azdina Jamal, Ibrahim Jantan, Nor Ashila Aladdin, Kartiniwati Muhammad, Fhataheya Buang, Hazni Falina Mohamad, Idin Sahidin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.

Original languageEnglish
Pages (from-to)3206-3220
Number of pages15
JournalMolecules
Volume20
Issue number2
DOIs
Publication statusPublished - 1 Feb 2015

Fingerprint

Dilleniaceae
prostaglandins
Dinoprostone
quantitative analysis
Inhibitory Concentration 50
Methanol
Plant Bark
High Pressure Liquid Chromatography
acids
Plant Roots
High performance liquid chromatography
Reverse-Phase Chromatography
Cyclooxygenase 2
Complex Mixtures
Chemical analysis
stems
Indomethacin
Radioimmunoassay
Lipopolysaccharides
Blood

Keywords

  • Dillenia serrata
  • Dilleniaceae
  • HPLC
  • Prostaglandin E
  • Triterpenoids

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Inhibitory effect of triterpenoids from Dillenia serrata (Dilleniaceae) on prostaglandin e2 production and quantitative HPLC analysis of its koetjapic acid and betulinic acid contents. / Jalil, Juriyati; Sabandar, Carla W.; Ahmat, Norizan; Jamal, Jamia Azdina; Jantan, Ibrahim; Aladdin, Nor Ashila; Muhammad, Kartiniwati; Buang, Fhataheya; Mohamad, Hazni Falina; Sahidin, Idin.

In: Molecules, Vol. 20, No. 2, 01.02.2015, p. 3206-3220.

Research output: Contribution to journalArticle

Jalil, Juriyati ; Sabandar, Carla W. ; Ahmat, Norizan ; Jamal, Jamia Azdina ; Jantan, Ibrahim ; Aladdin, Nor Ashila ; Muhammad, Kartiniwati ; Buang, Fhataheya ; Mohamad, Hazni Falina ; Sahidin, Idin. / Inhibitory effect of triterpenoids from Dillenia serrata (Dilleniaceae) on prostaglandin e2 production and quantitative HPLC analysis of its koetjapic acid and betulinic acid contents. In: Molecules. 2015 ; Vol. 20, No. 2. pp. 3206-3220.
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