Inhibitory and resistance-modifying potential of plant-based alkaloids against methicillin-resistant Staphylococcus aureus (MRSA)

Mastura Mohtar, Saiful Azmi Johari, Abdul Rashid Li, Mazurah Mohamed Isa, Shuhaimi Mustafa, Abdul Manaf Ali, Dayang Fredalina Basri

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Increased prevalence of methicillin-resistant Staphylococcus aureus (MRSA) has become a major threat to the health sector worldwide due to their virulence, limited therapeutic options and their distribution in both hospital and community settings. Discovery and development of new anti-MRSA agents as alternatives to the very few antibiotics left in the armamentarium are, thus, urgently required. Recently, an efflux mechanism in MRSA has been identified as one of the main contributors of resistance towards various structurally unrelated antibiotics. The potential of reserpine (a phytoalkaloid) as efflux pump inhibitor (EPI) against various microbes remains limited as the concentration needed for inhibition is toxic to humans. This study therefore aimed to evaluate 13 alkaloid compounds as potential inhibitory agents and/or potential EPIs against a panel of three MRSA isolates which not only differ in their susceptibility to vancomycin (amongst the last drugs available to treat serious MRSA infection), but also exhibited active efflux activity. Results indicated berberine's moderate inhibitiory activity against two MRSA isolates scoring a minimum inhibitory concentration (MIC) value of 125 μg/ml. Notable efflux inhibitory activity (ranging from two- to eightfold Ethidium Bromide MIC reduction) meanwhile was detected from quinine, piperine and harmaline using reserpine as the positive control. Findings from this study support the opinion that a vast number of potential phytocompounds with pharmacological potential await discovery. Therapeutic application of these compounds, however, warrants further investigation to ascertain their pharmacodynamics and safety aspects.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalCurrent Microbiology
Volume59
Issue number2
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Alkaloids
Reserpine
piperine
Microbial Sensitivity Tests
Harmaline
Anti-Bacterial Agents
Berberine
Quinine
Ethidium
Poisons
Vancomycin
Virulence
Pharmacology
Safety
Therapeutics
Infection
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Inhibitory and resistance-modifying potential of plant-based alkaloids against methicillin-resistant Staphylococcus aureus (MRSA). / Mohtar, Mastura; Johari, Saiful Azmi; Li, Abdul Rashid; Isa, Mazurah Mohamed; Mustafa, Shuhaimi; Ali, Abdul Manaf; Basri, Dayang Fredalina.

In: Current Microbiology, Vol. 59, No. 2, 08.2009, p. 181-186.

Research output: Contribution to journalArticle

Mohtar, Mastura ; Johari, Saiful Azmi ; Li, Abdul Rashid ; Isa, Mazurah Mohamed ; Mustafa, Shuhaimi ; Ali, Abdul Manaf ; Basri, Dayang Fredalina. / Inhibitory and resistance-modifying potential of plant-based alkaloids against methicillin-resistant Staphylococcus aureus (MRSA). In: Current Microbiology. 2009 ; Vol. 59, No. 2. pp. 181-186.
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