Inhibitory activities of compounds from the twigs of Garcinia hombroniana pierre on human low-density lipoprotein (LDL) oxidation and platelet aggregation

Fadlina Chany Saputri, Ibrahim Jantan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′-tetrahydroxy-4- methoxybenzophenone, 1,7-dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin-5-en-3β-ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper-mediated LDL oxidation and arachidonic acid (AA)-, adenosine diphosphate (ADP)-, collagen-induced platelet aggregation in vitro. Among the compounds tested, 3,5,3′,5′-tetrahydroxy-4-methoxybenzophenone and 1,7-dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half-maximal inhibitory concentration (IC50) values of 6.6 and 1.7 μm, respectively. 3,5,3′,5′-Tetrahydroxy-4-methoxybenzophenone exhibited strong activity on AA-, ADP- and collagen-induced platelet aggregation with IC50 values of 53.6, 125.7 and 178.6 μm, respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP-induced aggregation with IC50 value of 5.7 μm. Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP-induced platelet aggregation.

Original languageEnglish
Pages (from-to)1845-1850
Number of pages6
JournalPhytotherapy Research
Volume26
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Garcinia
Platelet Aggregation
LDL Lipoproteins
Adenosine Diphosphate
Inhibitory Concentration 50
Arachidonic Acid
Collagen
Stigmasterol
Methanol
Chromatography
Copper
4-methoxybenzophenone

Keywords

  • antiplatelet aggregation
  • benzophenone
  • Garcinia hombroniana Pierre (Guttiferae)
  • LDL antioxidation
  • xanthone

ASJC Scopus subject areas

  • Pharmacology

Cite this

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title = "Inhibitory activities of compounds from the twigs of Garcinia hombroniana pierre on human low-density lipoprotein (LDL) oxidation and platelet aggregation",
abstract = "The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′-tetrahydroxy-4- methoxybenzophenone, 1,7-dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin-5-en-3β-ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper-mediated LDL oxidation and arachidonic acid (AA)-, adenosine diphosphate (ADP)-, collagen-induced platelet aggregation in vitro. Among the compounds tested, 3,5,3′,5′-tetrahydroxy-4-methoxybenzophenone and 1,7-dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half-maximal inhibitory concentration (IC50) values of 6.6 and 1.7 μm, respectively. 3,5,3′,5′-Tetrahydroxy-4-methoxybenzophenone exhibited strong activity on AA-, ADP- and collagen-induced platelet aggregation with IC50 values of 53.6, 125.7 and 178.6 μm, respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP-induced aggregation with IC50 value of 5.7 μm. Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP-induced platelet aggregation.",
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author = "Saputri, {Fadlina Chany} and Ibrahim Jantan",
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N2 - The methanol extract of the twigs of Garcinia hombroniana, which showed strong LDL antioxidation and antiplatelet aggregation activities, was subjected to column chromatography to obtain 3,5,3′,5′-tetrahydroxy-4- methoxybenzophenone, 1,7-dihydroxyxanthone and eight triterpenoids, garcihombronane B, D, E and F, friedelin, glutin-5-en-3β-ol, stigmasterol and lupeol. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit copper-mediated LDL oxidation and arachidonic acid (AA)-, adenosine diphosphate (ADP)-, collagen-induced platelet aggregation in vitro. Among the compounds tested, 3,5,3′,5′-tetrahydroxy-4-methoxybenzophenone and 1,7-dihydroxyxanthone showed strong inhibitory activity on LDL oxidation with half-maximal inhibitory concentration (IC50) values of 6.6 and 1.7 μm, respectively. 3,5,3′,5′-Tetrahydroxy-4-methoxybenzophenone exhibited strong activity on AA-, ADP- and collagen-induced platelet aggregation with IC50 values of 53.6, 125.7 and 178.6 μm, respectively, while 1,7 dihydroxyxanthone showed significant and selective inhibitory activity against ADP-induced aggregation with IC50 value of 5.7 μm. Of the triterpenoids tested, garcihombronane B showed moderate activity against LDL oxidation and garcihombronane D and F showed selective inhibition on ADP-induced platelet aggregation.

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