Inhibition of macromolecular synthesis in cultured macrophages by Pseudomonas pseudomallei exotoxin

R. Mohamed, Sheila Nathan, Mohammed Noor Embi, N. Razak, G. Ismail

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Pseudomonas pseudomallei exotoxin was found to be a potent inhibitor of protein and DNA synthesis in cultured macrophages. Inhibition of DNA synthesis occurred at toxin concentrations as low as 1-2 μg/ml and inhibition of 3H-thymidine uptake was almost complete at concentrations of 8 μg/ml or more. A close correlation between cell damage and inhibition by DNA synthesis was observed. For protein synthesis, inhibition was obtained at much lower doses (0.06-2.0 μg/ml) of the toxin. At similar toxin concentrations, DNA synthesis was marginally affected. Further, it was shown that protein synthesis inhibition occurred almost immediately after incubation, reaching its maximal inhibitory effect of 70% after 6 hr. DNA synthesis, however, was minimally affected by a similar toxin concentration even after 10 hr of incubation. The inhibition of macromolecular synthesis in macrophages by P. pseudomallei exotoxin may be relevant to its modulatory effect on the host defense mechanism.

Original languageEnglish
Pages (from-to)811-820
Number of pages10
JournalMicrobiology and Immunology
Volume33
Issue number10
Publication statusPublished - 1989

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Burkholderia pseudomallei
Exotoxins
Macrophages
DNA
Nucleic Acid Synthesis Inhibitors
Protein Synthesis Inhibitors
Thymidine
DNA Damage
Proteins

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Inhibition of macromolecular synthesis in cultured macrophages by Pseudomonas pseudomallei exotoxin. / Mohamed, R.; Nathan, Sheila; Embi, Mohammed Noor; Razak, N.; Ismail, G.

In: Microbiology and Immunology, Vol. 33, No. 10, 1989, p. 811-820.

Research output: Contribution to journalArticle

Mohamed, R. ; Nathan, Sheila ; Embi, Mohammed Noor ; Razak, N. ; Ismail, G. / Inhibition of macromolecular synthesis in cultured macrophages by Pseudomonas pseudomallei exotoxin. In: Microbiology and Immunology. 1989 ; Vol. 33, No. 10. pp. 811-820.
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