Inhibition of human platelet aggregation and low-density lipoprotein oxidation by premna foetida extract and its major compounds

Roza Dianita, Ibrahim Jantan

Research output: Contribution to journalArticle

Abstract

Many Premna species have been used in traditional medicine to treat hypertension and cardiac insufficiency, and as a tonic for cardiac-related problems. Some have been reported to possess cardiovascular protective activity through several possible mechanisms, but not Premna foetida. In the present study, the methanol extract of P. foetida leaves (PFM) and its isolated compounds were evaluated for their ability to inhibit copper-mediated human low-density lipoprotein (LDL) oxidation and arachidonic acid (AA)- and adenosine diphosphate (ADP)-induced platelet aggregation. Six flavonoids, three triterpenoids, vanillic acid and stigmasterol were successfully isolated from PFM. Of the isolated compounds, quercetin was the most active against LDL oxidation (IC 50 4.25 µM). The flavonols were more active than the flavones against LDL oxidation, suggesting that hydroxyl group at C-3 and the catechol moiety at B-ring may play important roles in protecting LDL from oxidation. Most tested flavonoids showed stronger inhibition towards AA-induced than the ADP-induced platelet aggregation with apigenin exhibiting the strongest effect (IC 50 52.3 and 127.4 µM, respectively) while quercetin and kaempferol showed moderate activity. The results suggested that flavonoids, especially quercetin, apigenin and kaempferol were among the major constituents of P. foetida responsible for anti-LDL oxidation and anti-platelet aggregation.

Original languageEnglish
Article number1469
JournalMolecules
DOIs
Publication statusPublished - 13 Apr 2019
Externally publishedYes

Fingerprint

lipoproteins
Platelets
platelets
Platelet Aggregation
LDL Lipoproteins
Agglomeration
Quercetin
Oxidation
oxidation
pulse frequency modulation
Flavonoids
Apigenin
adenosine diphosphate
Arachidonic Acid
Adenosine Diphosphate
acids
Vanillic Acid
Stigmasterol
Flavones
hypertension

Keywords

  • Anti-LDL oxidation
  • Antiplatelet aggregation
  • Apigenin
  • Flavonoids
  • Premna foetida
  • Quercetin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Inhibition of human platelet aggregation and low-density lipoprotein oxidation by premna foetida extract and its major compounds. / Dianita, Roza; Jantan, Ibrahim.

In: Molecules, 13.04.2019.

Research output: Contribution to journalArticle

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