Inhibition of fouling bacteria by the marine epiphytes from selected locations in Malaysia

Nor Afifah Supardy, Darah Ibrahim, Sharifah Radziah Mat Nor, Wan Norhana Md Noordin, Ahmad Ismail

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The rising demand for natural antifouling agents has led to the search for potential secondary metabolites from marine epiphytic bacteria. The objective of this study is to isolate the epiphytic bacteria from various marine invertebrates and to screen for their antibacterial activities against a list of fouling bacteria. In this study, the antibacterial activity of 91 epiphytic bacteria were tested against 21 fouling bacteria. In the preliminary screening of the inhibition activity, the fouling bacteria were cross-streaked with the epiphytic bacteria. Isolates with prominent inhibitory activity (susceptible fouling bacteria > 40%) were subjected to fermentation for five days, and their cell-free supernatants were extracted with ethyl acetate (1:1 v/v) to obtain the crude extracts. In the secondary screening, the extracts were tested against fouling bacteria via the disc diffusion assay. At 2.00 mg/disc, 12 out of 30 extracts were effective against at least one fouling bacteria. Two most outstanding antibacterial activities were demonstrated by isolates PD4.8 and PD4.9, which were isolated from Caulerpa racemosa. Isolate PD4.8 was effective against five fouling bacteria with the largest inhibition zone of 16.0 mm, whilst PD4.9 was effective against four fouling bacteria with the largest inhibition zone of 15.0 mm. This study demonstrated a significant antibacterial activity of epiphytic isolates PD4.8 and PD4.9 against the fouling bacteria, and should be further investigated for production of antifouling compound.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalMalaysian Journal of Science
Volume36
Issue number2
Publication statusPublished - 31 Aug 2017

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fouling
bacterium
antifouling agent
antifouling
secondary metabolite
fermentation
acetate
invertebrate
assay

ASJC Scopus subject areas

  • General

Cite this

Supardy, N. A., Ibrahim, D., Nor, S. R. M., Md Noordin, W. N., & Ismail, A. (2017). Inhibition of fouling bacteria by the marine epiphytes from selected locations in Malaysia. Malaysian Journal of Science, 36(2), 17-21.

Inhibition of fouling bacteria by the marine epiphytes from selected locations in Malaysia. / Supardy, Nor Afifah; Ibrahim, Darah; Nor, Sharifah Radziah Mat; Md Noordin, Wan Norhana; Ismail, Ahmad.

In: Malaysian Journal of Science, Vol. 36, No. 2, 31.08.2017, p. 17-21.

Research output: Contribution to journalArticle

Supardy, NA, Ibrahim, D, Nor, SRM, Md Noordin, WN & Ismail, A 2017, 'Inhibition of fouling bacteria by the marine epiphytes from selected locations in Malaysia', Malaysian Journal of Science, vol. 36, no. 2, pp. 17-21.
Supardy, Nor Afifah ; Ibrahim, Darah ; Nor, Sharifah Radziah Mat ; Md Noordin, Wan Norhana ; Ismail, Ahmad. / Inhibition of fouling bacteria by the marine epiphytes from selected locations in Malaysia. In: Malaysian Journal of Science. 2017 ; Vol. 36, No. 2. pp. 17-21.
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