Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants

Ibrahim Jantan, Nurul Hikmah Harun, Abdi Wira Septama, Shahnaz Murad, Osman Mesaik Mohammed Ahmed Hassan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC 50 values ranging from 0.5 to 0.7 μg/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC50 values ranging from 0.9 to 1.5 μg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC50 valuescomparable to that of ibuprofen (1.5 μg/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents.

Original languageEnglish
Pages (from-to)400-405
Number of pages6
JournalJournal of Natural Medicines
Volume65
Issue number2
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Luminol
Chemiluminescence
Respiratory Burst
Phagocytes
Medicinal Plants
Luminescence
Macrophages
Inhibitory Concentration 50
Garcinia mangostana
Piper nigrum
Garcinia
Alpinia
Phyllanthus
Ginger
Curcuma
Ibuprofen
Cell Extracts
Innate Immunity
Cell Movement
Methanol

Keywords

  • Chemiluminescence
  • Chemotactic activity
  • Immunomodulatory effect
  • Macrophage cells
  • Medicinal plants
  • Polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Complementary and alternative medicine

Cite this

Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants. / Jantan, Ibrahim; Hikmah Harun, Nurul; Wira Septama, Abdi; Murad, Shahnaz; Mohammed Ahmed Hassan, Osman Mesaik.

In: Journal of Natural Medicines, Vol. 65, No. 2, 04.2011, p. 400-405.

Research output: Contribution to journalArticle

@article{7c577299ab2b4d638863e5d5ce713a44,
title = "Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants",
abstract = "The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC 50 values ranging from 0.5 to 0.7 μg/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC50 values ranging from 0.9 to 1.5 μg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC50 valuescomparable to that of ibuprofen (1.5 μg/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents.",
keywords = "Chemiluminescence, Chemotactic activity, Immunomodulatory effect, Macrophage cells, Medicinal plants, Polymorphonuclear leukocytes",
author = "Ibrahim Jantan and {Hikmah Harun}, Nurul and {Wira Septama}, Abdi and Shahnaz Murad and {Mohammed Ahmed Hassan}, {Osman Mesaik}",
year = "2011",
month = "4",
doi = "10.1007/s11418-010-0492-8",
language = "English",
volume = "65",
pages = "400--405",
journal = "Natural Medicines",
issn = "1861-0293",
publisher = "Springer Japan",
number = "2",

}

TY - JOUR

T1 - Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants

AU - Jantan, Ibrahim

AU - Hikmah Harun, Nurul

AU - Wira Septama, Abdi

AU - Murad, Shahnaz

AU - Mohammed Ahmed Hassan, Osman Mesaik

PY - 2011/4

Y1 - 2011/4

N2 - The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC 50 values ranging from 0.5 to 0.7 μg/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC50 values ranging from 0.9 to 1.5 μg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC50 valuescomparable to that of ibuprofen (1.5 μg/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents.

AB - The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC 50 values ranging from 0.5 to 0.7 μg/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC50 values ranging from 0.9 to 1.5 μg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC50 valuescomparable to that of ibuprofen (1.5 μg/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents.

KW - Chemiluminescence

KW - Chemotactic activity

KW - Immunomodulatory effect

KW - Macrophage cells

KW - Medicinal plants

KW - Polymorphonuclear leukocytes

UR - http://www.scopus.com/inward/record.url?scp=79956327205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956327205&partnerID=8YFLogxK

U2 - 10.1007/s11418-010-0492-8

DO - 10.1007/s11418-010-0492-8

M3 - Article

VL - 65

SP - 400

EP - 405

JO - Natural Medicines

JF - Natural Medicines

SN - 1861-0293

IS - 2

ER -