Inhibition of β-catenin and KRAS expressions by piper betle in azoxymethane-induced colon cancer of male fischer 344 rats

Faezah Esa, Wan Zurinah Wan Ngah, A. Rahman A Jama, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To investigate the chemopreventive effect of Piper betle (PB) on preneoplastic lesions (aberrant crypt foci [ACF]) induced by azoxymethane (AOM) in rats and its effect on colorectal cancer biomarkers (β - catenin, KRAS, p53 and p21). STUDY DESIGN: A total of 32 male Fischer 344 rats were divided into phase 1 and phase 2 groups (8 and 24 weeks of AOM administration, respectively). Each phase was divided into 4 groups: control or normal saline (NS) (1 mL/kg), AOM (15 mg/kg body weight, once weekly for 2 weeks), PB (75 mg/kg body weight) and AOM+PB. PB was force-fed to rats a week after the second dose of AOM and NS. The colon was cut open longitudinally for methylene blue and immunohistochemistry staining. RESULTS: AOM administration showed formation of ACF at 8 and 24 weeks. PB, however, did not reduce ACF formation at either week, but it managed to reduce β -catenin expression and KRAS found highly expressed in the AOM group of phase 1 rats. No immunoreactivities of p53 and p21 were detected in phase 2 rats, but instead inflammatory cells were visible in between the lesions. CONCLUSION: PB may act as a potential chemopreventive agent in the early stage of colon carcinogenesis by suppressing the expressions of β -catenin and KRAS.

Original languageEnglish
Pages (from-to)324-334
Number of pages11
JournalAnalytical and Quantitative Cytology and Histology
Volume35
Issue number6
Publication statusPublished - Dec 2013

Fingerprint

Piper betle
Azoxymethane
Catenins
Inbred F344 Rats
Colonic Neoplasms
Aberrant Crypt Foci
Colon
Body Weight
Methylene Blue
Tumor Biomarkers
Colorectal Neoplasms
Carcinogenesis
Immunohistochemistry
Staining and Labeling
Control Groups

Keywords

  • Azoxymethane
  • Beta-catenin
  • Chemoprevention
  • Chemoprophylaxis
  • Colon cancer
  • Ki-ras protein, rat
  • Piper betle
  • sarcospan (Kras oncogeneassociated gene) protein, human

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Inhibition of β-catenin and KRAS expressions by piper betle in azoxymethane-induced colon cancer of male fischer 344 rats. / Esa, Faezah; Wan Ngah, Wan Zurinah; Jama, A. Rahman A; Mohd Yusof, Yasmin Anum.

In: Analytical and Quantitative Cytology and Histology, Vol. 35, No. 6, 12.2013, p. 324-334.

Research output: Contribution to journalArticle

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abstract = "To investigate the chemopreventive effect of Piper betle (PB) on preneoplastic lesions (aberrant crypt foci [ACF]) induced by azoxymethane (AOM) in rats and its effect on colorectal cancer biomarkers (β - catenin, KRAS, p53 and p21). STUDY DESIGN: A total of 32 male Fischer 344 rats were divided into phase 1 and phase 2 groups (8 and 24 weeks of AOM administration, respectively). Each phase was divided into 4 groups: control or normal saline (NS) (1 mL/kg), AOM (15 mg/kg body weight, once weekly for 2 weeks), PB (75 mg/kg body weight) and AOM+PB. PB was force-fed to rats a week after the second dose of AOM and NS. The colon was cut open longitudinally for methylene blue and immunohistochemistry staining. RESULTS: AOM administration showed formation of ACF at 8 and 24 weeks. PB, however, did not reduce ACF formation at either week, but it managed to reduce β -catenin expression and KRAS found highly expressed in the AOM group of phase 1 rats. No immunoreactivities of p53 and p21 were detected in phase 2 rats, but instead inflammatory cells were visible in between the lesions. CONCLUSION: PB may act as a potential chemopreventive agent in the early stage of colon carcinogenesis by suppressing the expressions of β -catenin and KRAS.",
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