Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1

Roberto Magliozzi, Zunamys I. Carrero, Low Teck Yew, Laurensia Yuniati, Christian Valdes-Quezada, Flore Kruiswijk, Koen van Wijk, Albert J.R. Heck, Catherine L. Jackson, Daniele Guardavaccaro

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although much is known about how chromosome segregation is coupled to cell division, how intracellular organelles partition during mitotic division is poorly understood. We report that the phosphorylation-dependent degradation of the ARFGEF GBF1 regulates organelle trafficking during cell division. We show that, in mitosis, GBF1 is phosphorylated on Ser292 and Ser297 by casein kinase-2 allowing recognition by the F-box protein βTrCP. GBF1 interaction with βTrCP recruits GBF1 to the SCFβTrCP ubiquitin ligase complex, triggering its degradation. Phosphorylation and degradation of GBF1 occur along microtubules at the intercellular bridge of telophase cells and are required for Golgi membrane positioning and postmitotic Golgi reformation. Indeed, expression of a non-degradable GBF1 mutant inhibits the transport of the Golgi cluster adjacent to the midbody toward the Golgi twin positioned next to the centrosome and results in defective Golgi reassembly and cytokinesis failure. These findings define a mechanism that controls postmitotic Golgi reassembly and inheritance. Magliozzi et al. demonstrate that, in mitosis, the ARFGEF GBF1 is targeted for ubiquitin-dependent degradation by casein kinase-2 and the SCFβTrCP ubiquitin ligase and show that GBF1 proteolysis is required for Golgi inheritance and accurate cell division.

Original languageEnglish
Pages (from-to)3381-3391.e4
JournalCell Reports
Volume23
Issue number11
DOIs
Publication statusPublished - 12 Jun 2018
Externally publishedYes

Fingerprint

Cytokinesis
Golgi Apparatus
Ubiquitin
Cell Division
Casein Kinase II
Ligases
Mitosis
Degradation
Organelles
Phosphorylation
Cells
F-Box Proteins
Telophase
Centrosome
Chromosome Segregation
Proteolysis
Microtubules
Chromosomes
Membranes

Keywords

  • cell division
  • Cullin-RING ubiquitin ligase
  • cytokinesis
  • GBF1
  • Golgi apparatus
  • mitosis
  • protein degradation
  • ubiquitin-proteasome system

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Magliozzi, R., Carrero, Z. I., Teck Yew, L., Yuniati, L., Valdes-Quezada, C., Kruiswijk, F., ... Guardavaccaro, D. (2018). Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. Cell Reports, 23(11), 3381-3391.e4. https://doi.org/10.1016/j.celrep.2018.05.031

Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. / Magliozzi, Roberto; Carrero, Zunamys I.; Teck Yew, Low; Yuniati, Laurensia; Valdes-Quezada, Christian; Kruiswijk, Flore; van Wijk, Koen; Heck, Albert J.R.; Jackson, Catherine L.; Guardavaccaro, Daniele.

In: Cell Reports, Vol. 23, No. 11, 12.06.2018, p. 3381-3391.e4.

Research output: Contribution to journalArticle

Magliozzi, R, Carrero, ZI, Teck Yew, L, Yuniati, L, Valdes-Quezada, C, Kruiswijk, F, van Wijk, K, Heck, AJR, Jackson, CL & Guardavaccaro, D 2018, 'Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1', Cell Reports, vol. 23, no. 11, pp. 3381-3391.e4. https://doi.org/10.1016/j.celrep.2018.05.031
Magliozzi R, Carrero ZI, Teck Yew L, Yuniati L, Valdes-Quezada C, Kruiswijk F et al. Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. Cell Reports. 2018 Jun 12;23(11):3381-3391.e4. https://doi.org/10.1016/j.celrep.2018.05.031
Magliozzi, Roberto ; Carrero, Zunamys I. ; Teck Yew, Low ; Yuniati, Laurensia ; Valdes-Quezada, Christian ; Kruiswijk, Flore ; van Wijk, Koen ; Heck, Albert J.R. ; Jackson, Catherine L. ; Guardavaccaro, Daniele. / Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. In: Cell Reports. 2018 ; Vol. 23, No. 11. pp. 3381-3391.e4.
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