Inheritance of allozyme in Shorea Leprosula (Dipterocarpaceae)

S. L. Lee, R Wickneswari V Ratnam, M. C. Mahani, A. H. Zakri

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Thirty-four to one hundred seeds from 14 half-sib families of Shorea leprosula were analysed by starch gel electrophoresis in order to verify the genetic control and mode of inheritance of allozyme loci. Of 29 enzyme systems investigated, 11 (AAT, EST, GDH, GPI, IDH, MDH, ME, PGM, SDH, TPI and UGP) exhibited high enzyme activity. To avoid uncertainties in genotyping, enzyme systems from these which were ontogenetically unstable (GDH), tissue specific (AAT), or had complex banding patterns (EST, ME and TPI) were excluded in genetic analyses. Of the remaining 6 enzyme systems (GPI, IDH, MDH, PGM, SDH and UGP), 10 codominant allozyme loci (Gpi-2, Pgm, Idh, Mdh-1, Mdh-2, Mdh-3, Sdh-1, Sdh-2, Ugp-1 and Ugp-2) were intuitively postulated. Genetic analysis utilising single trees and their progenies supported the intuitive interpretation of all 10 proposed loci, with the exception of Mdh-1. For Mdh-3, since maternal heterozygous trees were unavailable, the hypothesis of genetic control must be considered as preliminary.

Original languageEnglish
Pages (from-to)124-138
Number of pages15
JournalJournal of Tropical Forest Science
Volume12
Issue number1
Publication statusPublished - Jan 2000

Fingerprint

Dipterocarpaceae
Shorea
allozyme
allozymes
Isoenzymes
inheritance (genetics)
enzyme
loci
Expressed Sequence Tags
Enzymes
enzymes
starch gels
genetic analysis
Starch Gel Electrophoresis
starch
gel electrophoresis
genotyping
enzyme activity
genetic techniques and protocols
electrokinesis

Keywords

  • Allozyme
  • Dipterocarpaceae
  • Malaysia
  • Mode of inheritance
  • Shorea leprosula
  • Starch gel electrophoresis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Inheritance of allozyme in Shorea Leprosula (Dipterocarpaceae). / Lee, S. L.; V Ratnam, R Wickneswari; Mahani, M. C.; Zakri, A. H.

In: Journal of Tropical Forest Science, Vol. 12, No. 1, 01.2000, p. 124-138.

Research output: Contribution to journalArticle

Lee, S. L. ; V Ratnam, R Wickneswari ; Mahani, M. C. ; Zakri, A. H. / Inheritance of allozyme in Shorea Leprosula (Dipterocarpaceae). In: Journal of Tropical Forest Science. 2000 ; Vol. 12, No. 1. pp. 124-138.
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AB - Thirty-four to one hundred seeds from 14 half-sib families of Shorea leprosula were analysed by starch gel electrophoresis in order to verify the genetic control and mode of inheritance of allozyme loci. Of 29 enzyme systems investigated, 11 (AAT, EST, GDH, GPI, IDH, MDH, ME, PGM, SDH, TPI and UGP) exhibited high enzyme activity. To avoid uncertainties in genotyping, enzyme systems from these which were ontogenetically unstable (GDH), tissue specific (AAT), or had complex banding patterns (EST, ME and TPI) were excluded in genetic analyses. Of the remaining 6 enzyme systems (GPI, IDH, MDH, PGM, SDH and UGP), 10 codominant allozyme loci (Gpi-2, Pgm, Idh, Mdh-1, Mdh-2, Mdh-3, Sdh-1, Sdh-2, Ugp-1 and Ugp-2) were intuitively postulated. Genetic analysis utilising single trees and their progenies supported the intuitive interpretation of all 10 proposed loci, with the exception of Mdh-1. For Mdh-3, since maternal heterozygous trees were unavailable, the hypothesis of genetic control must be considered as preliminary.

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