Influences of explant type on in vitro regeneration of Malaysian Chilli (Capsicum annuum L.) var CB 4

Z. Alizah, Zamri Zainal

Research output: Contribution to journalArticle

Abstract

Regeneration system via organogenesis has been developed for Malaysian chilli (Capsicum annuum L) var. CB4. Hypocotyls, cotyledons and petioles from In vitro germinated seedlings were cultured on medium comprising of Murashige and Skoog’s basal medium supplemented with 5 mg/l BAP, 1 mg/l IAA and 25 g/L DJ nutrient. Hypocotyls and petioles were found to be the best explants, based on its ability to produce shoot buds compared to cotyledons. Shoot buds were elongated on MS medium containing 3 mg/l BAP, 1 mg/l IAA, 15 g/L DJ, 2 mg/L GA3, 10 mg/l AgNO3 and 15 g/L DJ nutrient. Rooting was induced on MS basal containing IAA.

Original languageEnglish
Article numberARRB.30238
JournalAnnual Research and Review in Biology
Volume11
Issue number4
DOIs
Publication statusPublished - 2016

Fingerprint

Hypocotyl
Capsicum
in vitro regeneration
Cotyledon
petioles
Capsicum annuum
hypocotyls
Nutrients
cotyledons
Regeneration
explants
buds
Food
shoots
Organogenesis
nutrients
organogenesis
Seedlings
rooting
seedlings

Keywords

  • Capsicum annuum
  • Chilli
  • Explants
  • Plant regeneration
  • Tissue culture

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Influences of explant type on in vitro regeneration of Malaysian Chilli (Capsicum annuum L.) var CB 4. / Alizah, Z.; Zainal, Zamri.

In: Annual Research and Review in Biology, Vol. 11, No. 4, ARRB.30238, 2016.

Research output: Contribution to journalArticle

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