Influence of N-limitation on malic enzyme isoforms and lipogenesis of Cunninghamella bainieri 2A1

Aidil Abdul Hamid, Shuwahida Shuib, Ekhlass M. Taha, Othman Omar, Mohd Sahaid Khalil, Abdul Jalil Abdul Kader, Mohd Hafez Mohd Isa

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3 Citations (Scopus)

Abstract

The influence of the presence of ammonium ions in growth culture on malic enzyme (ME) isoforms activity and lipogenesis in Cunninghamella bainieri 2A1 was investigated. The fungus was cultivated in a nitrogen-limiting medium for 120 h at 30°C under two conditions. One of the cultures was intermittently fed with ammonium tartrate to maintain the ammonium concentrations above 0.5 g/L. The second culture was performed without any feeding to allow N limitation, thus promoting lipid accumulation. Activity staining of ME isoforms was carried out for both cultures. The culture which was not intermittently fed with ammonium tartrate achieved a maximum lipid content of 35% (g/g biomass) at 48 h. This culture possessed five ME isoforms (A, B, C, D and E) with isoform E showing a parallel correlation to lipid accumulation profile. In contrast, intensity of bands representing isoform D decreased as lipid accumulated. No appreciable differences of all other isoforms were observed. However, the culture which was intermittently fed with ammonium tartrate, accumulated only up to 16% lipid (g/g biomass). All isoforms were present but with a more pronounced activity of isoform D and a lower activity of isoform E was observed. These findings support further evidence that isoform E is the key isoform for lipid synthesis in C. bainieri 2A1.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJurnal Teknologi (Sciences and Engineering)
Volume67
Issue number1
DOIs
Publication statusPublished - Mar 2014

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Lipids
Enzymes
Biomass
Enzyme activity
Fungi
Lipogenesis
Nitrogen
Ions

Keywords

  • Lipid biosynthesis
  • Malic enzyme isoforms
  • Nitrogen controlled-media

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Influence of N-limitation on malic enzyme isoforms and lipogenesis of Cunninghamella bainieri 2A1. / Abdul Hamid, Aidil; Shuib, Shuwahida; Taha, Ekhlass M.; Omar, Othman; Khalil, Mohd Sahaid; Kader, Abdul Jalil Abdul; Isa, Mohd Hafez Mohd.

In: Jurnal Teknologi (Sciences and Engineering), Vol. 67, No. 1, 03.2014, p. 1-5.

Research output: Contribution to journalArticle

Abdul Hamid, Aidil ; Shuib, Shuwahida ; Taha, Ekhlass M. ; Omar, Othman ; Khalil, Mohd Sahaid ; Kader, Abdul Jalil Abdul ; Isa, Mohd Hafez Mohd. / Influence of N-limitation on malic enzyme isoforms and lipogenesis of Cunninghamella bainieri 2A1. In: Jurnal Teknologi (Sciences and Engineering). 2014 ; Vol. 67, No. 1. pp. 1-5.
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