Influence of gamma irradiation on polymerization of pyrrole and glucose oxidase immobilization onto poly (pyrrole)/poly (vinyl alcohol) matrix

Sarada Idris, Ahmad Ashrif A Bakar, Chantara Thevy Ratnam, Nur Hasiba Kamaruddin, Sahbudin Shaari

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This paper describes the immobilization of glucose oxidase, GOx onto polymer matrix comprising of poly(pyrrole), PPy and poly(vinyl alcohol), PVA using gamma irradiation technique. Py/PVA-GOx film was prepared by spreading PVA:GOx, 1:1 solution onto dried pyrrole film and exposed to gamma irradiation from cobalt 60 source at doses ranging from 0 to 60 kGy. The films were subjected to structural and morphological analyses by using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Scanning electron microscope (SEM), Field emission scanning electron microscope (FESEM) and Atomic-force microscopy (AFM) techniques. Similar studies were also made on pristine pyrrole film which served as control. The SEM and FTIR spectra of Py/PVA-GOx film revealed that pyrrole has been successfully polymerized through irradiation-induced reactions. The results on the morphological properties of the samples characterize using FESEM, SEM and AFM further confirmed the occurrence of radiation-induced modification of Py/PVA-GOx film. The FTIR spectra showed the existence of intermolecular interaction between polymer matrix and GOx indicating that GOx had been successfully immobilized onto Ppy/PVA matrix by radiation-induced reactions. Results revealed that radiation induced reactions such as polymerization of pyrrole, crosslinking of PVA, grafting between the adjacent PVA and pyrrole molecules as well as immobilization of GOx onto Ppy/PVA matrix occurred simultaneously upon gamma irradiation. The optimum dose for GOx immobilization in the polymer matrix found to be 40 kGy. Therefore it is clear that this irradiation technique offered a simple single process to produce Py/PVA-GOx film without additional crosslinking and polymerization agents.

Original languageEnglish
Pages (from-to)118-128
Number of pages11
JournalApplied Surface Science
Volume400
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Glucose Oxidase
Pyrroles
Glucose oxidase
Alcohols
Polymerization
Irradiation
Electron microscopes
Scanning
Polymer matrix
Fourier transform infrared spectroscopy
Radiation
Field emission
Crosslinking
Dosimetry
Atomic force microscopy
Cobalt
X ray photoelectron spectroscopy
Molecules

Keywords

  • Gamma irradiation
  • Glucose oxidase
  • Immobilization
  • Pyrrole polymerization

ASJC Scopus subject areas

  • Surfaces, Coatings and Films

Cite this

Influence of gamma irradiation on polymerization of pyrrole and glucose oxidase immobilization onto poly (pyrrole)/poly (vinyl alcohol) matrix. / Idris, Sarada; A Bakar, Ahmad Ashrif; Thevy Ratnam, Chantara; Kamaruddin, Nur Hasiba; Shaari, Sahbudin.

In: Applied Surface Science, Vol. 400, 01.04.2017, p. 118-128.

Research output: Contribution to journalArticle

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