Influence of chondroitin 4-sulphate on properties and cell behaviour of collagen hydrogel

Masrina Mohd Nadzir, Lau Sin Mun, Farah Dhaniyah Anour, Shiplu Roy Chowdhury, Mohd Fauzi Mh Busra

Research output: Contribution to journalArticle

Abstract

In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore, thermal stability was higher by 5°C in the presence of C4S. Human dermal fibroblasts were cultured and bioactivity of collagen/C4S hydrogel scaffolds was assayed. Although affinity of cell attachment was reduced on hydrogel scaffold with C4S, cells were able to spread well and displayed stretched morphology. Cell viability assay was performed to evaluate cell proliferation on collagen/C4S hydrogel scaffold. The results confirmed the inclusion of C4S to collagen improved the properties of hydrogel scaffold without affecting the growth rate of cells.

Original languageEnglish
Pages (from-to)242-250
Number of pages9
JournalInternational Journal of Nano and Biomaterials
Volume7
Issue number3
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Chondroitin Sulfates
Hydrogel
collagens
Collagen
Hydrogels
sulfates
Scaffolds
Scaffolds (biology)
cells
Sulfates
fibroblasts
Cell proliferation
Fibroblasts
Bioactivity
viability
swelling
attachment
Swelling
affinity
Assays

Keywords

  • Biomaterials
  • Characterisation
  • Chondroitin 4-sulphate
  • Collagen
  • Hydrogel scaffold
  • In vitro

ASJC Scopus subject areas

  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Physical and Theoretical Chemistry

Cite this

Influence of chondroitin 4-sulphate on properties and cell behaviour of collagen hydrogel. / Nadzir, Masrina Mohd; Mun, Lau Sin; Anour, Farah Dhaniyah; Chowdhury, Shiplu Roy; Busra, Mohd Fauzi Mh.

In: International Journal of Nano and Biomaterials, Vol. 7, No. 3, 01.01.2018, p. 242-250.

Research output: Contribution to journalArticle

Nadzir, Masrina Mohd ; Mun, Lau Sin ; Anour, Farah Dhaniyah ; Chowdhury, Shiplu Roy ; Busra, Mohd Fauzi Mh. / Influence of chondroitin 4-sulphate on properties and cell behaviour of collagen hydrogel. In: International Journal of Nano and Biomaterials. 2018 ; Vol. 7, No. 3. pp. 242-250.
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