Abstract
In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore, thermal stability was higher by 5°C in the presence of C4S. Human dermal fibroblasts were cultured and bioactivity of collagen/C4S hydrogel scaffolds was assayed. Although affinity of cell attachment was reduced on hydrogel scaffold with C4S, cells were able to spread well and displayed stretched morphology. Cell viability assay was performed to evaluate cell proliferation on collagen/C4S hydrogel scaffold. The results confirmed the inclusion of C4S to collagen improved the properties of hydrogel scaffold without affecting the growth rate of cells.
| Original language | English |
|---|---|
| Pages (from-to) | 242-250 |
| Number of pages | 9 |
| Journal | International Journal of Nano and Biomaterials |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
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Keywords
- Biomaterials
- Characterisation
- Chondroitin 4-sulphate
- Collagen
- Hydrogel scaffold
- In vitro
ASJC Scopus subject areas
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
- Physical and Theoretical Chemistry
Cite this
Influence of chondroitin 4-sulphate on properties and cell behaviour of collagen hydrogel. / Nadzir, Masrina Mohd; Mun, Lau Sin; Anour, Farah Dhaniyah; Chowdhury, Shiplu Roy; Busra, Mohd Fauzi Mh.
In: International Journal of Nano and Biomaterials, Vol. 7, No. 3, 01.01.2018, p. 242-250.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of chondroitin 4-sulphate on properties and cell behaviour of collagen hydrogel
AU - Nadzir, Masrina Mohd
AU - Mun, Lau Sin
AU - Anour, Farah Dhaniyah
AU - Chowdhury, Shiplu Roy
AU - Busra, Mohd Fauzi Mh
PY - 2018/1/1
Y1 - 2018/1/1
N2 - In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore, thermal stability was higher by 5°C in the presence of C4S. Human dermal fibroblasts were cultured and bioactivity of collagen/C4S hydrogel scaffolds was assayed. Although affinity of cell attachment was reduced on hydrogel scaffold with C4S, cells were able to spread well and displayed stretched morphology. Cell viability assay was performed to evaluate cell proliferation on collagen/C4S hydrogel scaffold. The results confirmed the inclusion of C4S to collagen improved the properties of hydrogel scaffold without affecting the growth rate of cells.
AB - In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore, thermal stability was higher by 5°C in the presence of C4S. Human dermal fibroblasts were cultured and bioactivity of collagen/C4S hydrogel scaffolds was assayed. Although affinity of cell attachment was reduced on hydrogel scaffold with C4S, cells were able to spread well and displayed stretched morphology. Cell viability assay was performed to evaluate cell proliferation on collagen/C4S hydrogel scaffold. The results confirmed the inclusion of C4S to collagen improved the properties of hydrogel scaffold without affecting the growth rate of cells.
KW - Biomaterials
KW - Characterisation
KW - Chondroitin 4-sulphate
KW - Collagen
KW - Hydrogel scaffold
KW - In vitro
UR - http://www.scopus.com/inward/record.url?scp=85052701265&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052701265&partnerID=8YFLogxK
U2 - 10.1504/IJNBM.2018.094252
DO - 10.1504/IJNBM.2018.094252
M3 - Article
AN - SCOPUS:85052701265
VL - 7
SP - 242
EP - 250
JO - International Journal of Nano and Biomaterials
JF - International Journal of Nano and Biomaterials
SN - 1752-8933
IS - 3
ER -