Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release

Mothanna Sadiq Al-Qubaisi, Abdullah Rasedee, Moayad Husein Flaifel, Sahrim Ahmad, Samer Hussein-Al-Ali, Mohd Zobir Hussein, Zulkarnain Zainal, Fatah H. Alhassan, Yun H. Taufiq-Yap, Eltayeb E M Eid, Ismail Adam Arbab, Bandar A. Al-Asbahi, Thomas J. Webster, Mohamed Ezzat El Zowalaty

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2±29.8nm, 0.524 ±0.013, and -60±14mV, respectively. We showed that NiZn ferrite nanoparticles had selective toxicity towards MCF-7, HepG2, and HT29cells, with a lesser effect on normal MCF 10A cells. The quantity of Bcl-2, Bax, p53, and cytochrome C in the cell lines mentioned above was determined by colorimetric methods in order to clarify the mechanism of action of NiZn ferrite nanoparticles in the killing of cancer cells. Our results indicate that NiZn ferrite nanoparticles promote apoptosis in cancer cells via caspase-3 and caspase-9, downregulation of Bcl-2, and upregulation of Bax and p53, with cytochrome C translocation. There was a concomitant collapse of the mitochondrial membrane potential in these cancer cells when treated with NiZn ferrite nanoparticles. This study shows that NiZn ferrite nanoparticles induce glutathione depletion in cancer cells, which results in increased production of reactive oxygen species and eventually, death of cancer cells.

Original languageEnglish
Pages (from-to)4115-4130
Number of pages16
JournalInternational Journal of Nanomedicine
Volume8
DOIs
Publication statusPublished - 29 Oct 2013

Fingerprint

Cell death
Cytochromes
Nanoparticles
Ferrite
Cells
Apoptosis
Proteins
Neoplasms
Caspase 9
Mitochondrial Membrane Potential
Polydispersity
Zeta potential
Hydrodynamics
ferrite
Caspase 3
Glutathione
Toxicity
Reactive Oxygen Species
Suspensions
Cell Death

Keywords

  • Cancer cells
  • Cytochrome C
  • Mitochondrial membrane potential
  • NiZn ferrite nanoparticles
  • p53
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Al-Qubaisi, M. S., Rasedee, A., Flaifel, M. H., Ahmad, S., Hussein-Al-Ali, S., Hussein, M. Z., ... El Zowalaty, M. E. (2013). Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release. International Journal of Nanomedicine, 8, 4115-4130. https://doi.org/10.2147/IJN.S50061

Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release. / Al-Qubaisi, Mothanna Sadiq; Rasedee, Abdullah; Flaifel, Moayad Husein; Ahmad, Sahrim; Hussein-Al-Ali, Samer; Hussein, Mohd Zobir; Zainal, Zulkarnain; Alhassan, Fatah H.; Taufiq-Yap, Yun H.; Eid, Eltayeb E M; Arbab, Ismail Adam; Al-Asbahi, Bandar A.; Webster, Thomas J.; El Zowalaty, Mohamed Ezzat.

In: International Journal of Nanomedicine, Vol. 8, 29.10.2013, p. 4115-4130.

Research output: Contribution to journalArticle

Al-Qubaisi, MS, Rasedee, A, Flaifel, MH, Ahmad, S, Hussein-Al-Ali, S, Hussein, MZ, Zainal, Z, Alhassan, FH, Taufiq-Yap, YH, Eid, EEM, Arbab, IA, Al-Asbahi, BA, Webster, TJ & El Zowalaty, ME 2013, 'Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release', International Journal of Nanomedicine, vol. 8, pp. 4115-4130. https://doi.org/10.2147/IJN.S50061
Al-Qubaisi, Mothanna Sadiq ; Rasedee, Abdullah ; Flaifel, Moayad Husein ; Ahmad, Sahrim ; Hussein-Al-Ali, Samer ; Hussein, Mohd Zobir ; Zainal, Zulkarnain ; Alhassan, Fatah H. ; Taufiq-Yap, Yun H. ; Eid, Eltayeb E M ; Arbab, Ismail Adam ; Al-Asbahi, Bandar A. ; Webster, Thomas J. ; El Zowalaty, Mohamed Ezzat. / Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release. In: International Journal of Nanomedicine. 2013 ; Vol. 8. pp. 4115-4130.
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