Induction of a putative metallothionein gene in the blood cockle, Anadara granosa, exposed to cadmium

M. K. Chan, Roohaida Othman, D. Zubir, S. Salmijah

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The relationship between a putative metallothionein gene (MT) and exposure to cadmium (Cd) in blood cockles (Anadara granosa) is reported. In a 96-h dose-response experiment, mortality of cockles was found to proportionately increase in the range of 0.2-5.0 mg/l Cd with a calculated LC50 of 2.94 mg/l. Exposure to 0.25 mg/l Cd for 16 days caused significant increases (P<0.05) in Cd concentrations in whole tissues, gills and hepatopancreas, and the accumulation of Cd in these tissues increased with the duration of exposure. Two cDNA libraries constructed using the hepatopancreas from control and Cd-treated cockles gave titres of 5.62×105 and 1.94×105 pfu/μg vector, respectively. A putative MT gene, AnaMT, of 510 nucleotides in length, was isolated from the treated cDNA library using a heterologous probe MT20 from the blue mussel, Mytilus edulis. Northern analyses using AnaMT as a probe indicated low expression of the MT mRNA in control animals. In cockles treated with 0.25 mg/l Cd for 4 days, MT mRNA level increased to approximately 168%, but declined to 108% at day 8. After 12 and 16 days of Cd treatment, expression of the MT gene was 138% and 187%, respectively, compared to the controls. These observations suggest that induction of the MT gene by a sublethal dose of Cd is rapid, occurring within 4 days of treatment.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume131
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Arcidae
Cardiidae
Metallothionein
Cadmium
Blood
Genes
Mytilus edulis
Hepatopancreas
Gene Library
Tissue
Gene Expression
Messenger RNA
Animals

Keywords

  • Anadara granosa
  • Bioindicator
  • Biomarker
  • Cadmium
  • Heavy metals
  • Induction
  • Metallothioneins
  • Over-expression

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Induction of a putative metallothionein gene in the blood cockle, Anadara granosa, exposed to cadmium. / Chan, M. K.; Othman, Roohaida; Zubir, D.; Salmijah, S.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 131, No. 2, 2002, p. 123-132.

Research output: Contribution to journalArticle

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