Induction and expression of cutinase activity during saprophytic growth of the fungal plant pathogen, Glomerella cingulata.

Farah Diba Abu Bakar, Abdul Munir Abd. Murad, Aidil Abdul Hamid, Zulkeflie Zamrod, Nor Muhammad Mahadi, Patrick Sullivan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The fungus Glomerella cingulata damages a wide range of crops in the tropics and subtropics. This fungus produces an extracellular enzyme, cutinase, which has been implicated in the plant disease process. A cutin monomer, 16-hydroxyhexadecanoic acid was used to induce cutinolytic esterase activity during saprophytic growth of G. cingulata. Cutinolytic esterase was induced in cultures grown in cutin monomer but was repressed by glucose. The pattern of cutinolytic activity obtained from the induction and enzyme assays correlates with the results of the Northern blot analyses. The cutinolytic activity and expression from this study demonstrates that the enzyme is tightly regulated by the cutin monomer, 16-hydroxyhexadecanoic acid.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalAsia-Pacific Journal of Molecular Biology and Biotechnology
Volume13
Issue number2
Publication statusPublished - 2005

Fingerprint

Phyllachorales
Esterases
Fungi
Growth
Plant Diseases
Enzyme Assays
Enzymes
Northern Blotting
Glucose
cutin
cutinase
16-hydroxypalmitic acid

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

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abstract = "The fungus Glomerella cingulata damages a wide range of crops in the tropics and subtropics. This fungus produces an extracellular enzyme, cutinase, which has been implicated in the plant disease process. A cutin monomer, 16-hydroxyhexadecanoic acid was used to induce cutinolytic esterase activity during saprophytic growth of G. cingulata. Cutinolytic esterase was induced in cultures grown in cutin monomer but was repressed by glucose. The pattern of cutinolytic activity obtained from the induction and enzyme assays correlates with the results of the Northern blot analyses. The cutinolytic activity and expression from this study demonstrates that the enzyme is tightly regulated by the cutin monomer, 16-hydroxyhexadecanoic acid.",
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AU - Abd. Murad, Abdul Munir

AU - Abdul Hamid, Aidil

AU - Zamrod, Zulkeflie

AU - Mahadi, Nor Muhammad

AU - Sullivan, Patrick

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AB - The fungus Glomerella cingulata damages a wide range of crops in the tropics and subtropics. This fungus produces an extracellular enzyme, cutinase, which has been implicated in the plant disease process. A cutin monomer, 16-hydroxyhexadecanoic acid was used to induce cutinolytic esterase activity during saprophytic growth of G. cingulata. Cutinolytic esterase was induced in cultures grown in cutin monomer but was repressed by glucose. The pattern of cutinolytic activity obtained from the induction and enzyme assays correlates with the results of the Northern blot analyses. The cutinolytic activity and expression from this study demonstrates that the enzyme is tightly regulated by the cutin monomer, 16-hydroxyhexadecanoic acid.

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