Induction and analysis of the alkaloid mitragynine content of a mitragyna speciosa suspension culture system upon elicitation and precursor feeding

Nor Nahazima Mohamad Zuldin, Ikram Md Said, Normah Mohd. Noor, Zamri Zainal, Chew Jin Kiat, Ismanizan Ismail

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture. The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4 mg L-1 2, 4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establish Mitragyna speciosa cell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3 mg L-1 2,4-D and 3% sucrose (9.47±0.4667 mL). The treatment of cultures with different concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250 mg L-1 yeast extract (9.275±0.082 mg L-1) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors; the highest level of mitragynine production was achieved in cultures treated with 3 M tryptophan and harvested at 6 days (13.226±1.98 mg L-1).

Original languageEnglish
Article number209434
JournalThe Scientific World Journal
Volume2013
DOIs
Publication statusPublished - 2013

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Mitragyna
2,4-Dichlorophenoxyacetic Acid
alkaloid
Alkaloids
Suspensions
Bony Callus
2,4 dichlorophenoxyacetic acid
N-benzyladenine
Salicylic Acid
salicylic acid
Tryptophan
Yeast
Yeasts
yeast
Kinetin
Plant Growth Regulators
Cell Size
Sucrose
sucrose
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Induction and analysis of the alkaloid mitragynine content of a mitragyna speciosa suspension culture system upon elicitation and precursor feeding. / Mohamad Zuldin, Nor Nahazima; Said, Ikram Md; Mohd. Noor, Normah; Zainal, Zamri; Jin Kiat, Chew; Ismail, Ismanizan.

In: The Scientific World Journal, Vol. 2013, 209434, 2013.

Research output: Contribution to journalArticle

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