Inducible repression of multiple expansin genes leads to growth suppression during leaf development

Hoe Han Goh, Jennifer Sloan, Carmen Dorca-Fornell, Andrew Fleming

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Expansins are cell wall proteins implicated in the control of plant growth via loosening of the extracellular matrix. They are encoded by a large gene family, and data linked to loss of single gene function to support a role of expansins in leaf growth remain limited. Here, we provide a quantitative growth analysis of transgenics containing an inducible artificial microRNA construct designed to down-regulate the expression of a number of expansin genes that an expression analysis indicated are expressed during the development of Arabidopsis (Arabidopsis thaliana) leaf 6. The results support the hypothesis that expansins are required for leaf growth and show that decreased expansin gene expression leads to a more marked repression of growth during the later stage of leaf development. In addition, a histological analysis of leaves in which expansin gene expression was suppressed indicates that, despite smaller leaves, mean cell size was increased. These data provide functional evidence for a role of expansins in leaf growth, indicate the importance of tissue/organ developmental context for the outcome of altered expansin gene expression, and highlight the separation of the outcome of expansin gene expression at the cellular and organ levels.

Original languageEnglish
Pages (from-to)1759-1770
Number of pages12
JournalPlant Physiology
Volume159
Issue number4
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

Fingerprint

expansins
leaf development
Gene Expression
Growth
Genes
genes
gene expression
Arabidopsis
leaves
MicroRNAs
Cell Size
plant growth control
Cell Wall
Extracellular Matrix
Down-Regulation
microRNA
extracellular matrix
Arabidopsis thaliana
cell walls
genetically modified organisms

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Inducible repression of multiple expansin genes leads to growth suppression during leaf development. / Goh, Hoe Han; Sloan, Jennifer; Dorca-Fornell, Carmen; Fleming, Andrew.

In: Plant Physiology, Vol. 159, No. 4, 08.2012, p. 1759-1770.

Research output: Contribution to journalArticle

Goh, Hoe Han ; Sloan, Jennifer ; Dorca-Fornell, Carmen ; Fleming, Andrew. / Inducible repression of multiple expansin genes leads to growth suppression during leaf development. In: Plant Physiology. 2012 ; Vol. 159, No. 4. pp. 1759-1770.
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