Indirect hemagglutination antibodies against Burkholderia pseudomallei in normal blood donors and suspected cases of melioidosis in Malaysia

A. Norazah, M. Y. Rohani, P. T. Chang, Mohamed Kamel Abdul Ghani

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7 Citations (Scopus)

Abstract

Interpretation of the indirect hemagglutination test (IHA) for melioidosis in endemic areas is difficult because of the presence of antibodies in apparently healthy individuals. Fifty-three out of 200 healthy blood donors in Malaysia showed positive antibody titers (≥ 1 : 40) against Burkholderia pseudomallei. Seven percent had an IHA titer of 1 : 40, 11% had an IHA titer of 1 : 80 while 8.5% had a titer ≥ 1 : 160. Out of 258 sera sent for melioidosis serology, 7% of the patients had an IHA titer of 1 : 40, 9% had an IHA titer of 1 : 80 while 20% had an IHA titer of ≥ 1 : 160. If a titer of ≥ 1 : 80 is taken as cut off point for positivity, 29% of the patients had positive melioidosis serology. Increasing the positivity threshold may jeopardize the sensitivity of the test. A more specific and sensitive test is needed.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume27
Issue number2
Publication statusPublished - Jun 1996

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Melioidosis
Burkholderia pseudomallei
Hemagglutination Tests
Malaysia
Hemagglutination
Blood Donors
Antibodies
Serology
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Interpretation of the indirect hemagglutination test (IHA) for melioidosis in endemic areas is difficult because of the presence of antibodies in apparently healthy individuals. Fifty-three out of 200 healthy blood donors in Malaysia showed positive antibody titers (≥ 1 : 40) against Burkholderia pseudomallei. Seven percent had an IHA titer of 1 : 40, 11{\%} had an IHA titer of 1 : 80 while 8.5{\%} had a titer ≥ 1 : 160. Out of 258 sera sent for melioidosis serology, 7{\%} of the patients had an IHA titer of 1 : 40, 9{\%} had an IHA titer of 1 : 80 while 20{\%} had an IHA titer of ≥ 1 : 160. If a titer of ≥ 1 : 80 is taken as cut off point for positivity, 29{\%} of the patients had positive melioidosis serology. Increasing the positivity threshold may jeopardize the sensitivity of the test. A more specific and sensitive test is needed.",
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AU - Abdul Ghani, Mohamed Kamel

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