Incorporation of zinc oxide nanoparticles into chitosan-collagen 3D porous scaffolds

Effect on morphology, mechanical properties and cytocompatibility of 3D porous scaffolds

Saleem Ullah, Ismail Zainol, Ruszymah Idrus

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The zinc oxide nanoparticles (particles size <50 nm) incorporated into chitosan-collagen 3D porous scaffolds and investigated the effect of zinc oxide nanoparticles incorporation on microstructure, mechanical properties, biodegradation and cytocompatibility of 3D porous scaffolds. The 0.5%, 1.0%, 2.0% and 4.0% zinc oxide nanoparticles chitosan-collagen 3D porous scaffolds were fabricated via freeze-drying technique. The zinc oxide nanoparticles incorporation effects consisting in chitosan-collagen 3D porous scaffolds were investigated by mechanical and swelling tests, and effect on the morphology of scaffolds examined microscopically. The biodegradation and cytocompatibility tests were used to investigate the effects of zinc oxide nanoparticles incorporation on the ability of scaffolds to use for tissue engineering application. The mean pore size and swelling ratio of scaffolds were decreased upon incorporation of zinc oxide nanoparticles however, the porosity, tensile modulus and biodegradation rate were increased upon incorporation of zinc oxide nanoparticles. In vitro culture of human fibroblasts and keratinocytes showed that the zinc oxide nanoparticles facilitated cell adhesion, proliferation and infiltration of chitosan-collagen 3D porous scaffolds. It was found that the zinc oxide nanoparticles incorporation enhanced porosity, tensile modulus and cytocompatibility of chitosan-collagen 3D porous scaffolds.

Original languageEnglish
Pages (from-to)1020-1029
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume104
DOIs
Publication statusPublished - 1 Nov 2017

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Zinc Oxide
Chitosan
Scaffolds
Nanoparticles
Collagen
Mechanical properties
Biodegradation
Porosity
Swelling
Elastic moduli
Freeze Drying
Cell adhesion
Fibroblasts
Tissue Engineering
Scaffolds (biology)
Keratinocytes
Tissue engineering
Cell culture
Particle Size
Infiltration

Keywords

  • Chitosan
  • Collagen
  • Cytocompatibility
  • Nanoparticles
  • Porous scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

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title = "Incorporation of zinc oxide nanoparticles into chitosan-collagen 3D porous scaffolds: Effect on morphology, mechanical properties and cytocompatibility of 3D porous scaffolds",
abstract = "The zinc oxide nanoparticles (particles size <50 nm) incorporated into chitosan-collagen 3D porous scaffolds and investigated the effect of zinc oxide nanoparticles incorporation on microstructure, mechanical properties, biodegradation and cytocompatibility of 3D porous scaffolds. The 0.5{\%}, 1.0{\%}, 2.0{\%} and 4.0{\%} zinc oxide nanoparticles chitosan-collagen 3D porous scaffolds were fabricated via freeze-drying technique. The zinc oxide nanoparticles incorporation effects consisting in chitosan-collagen 3D porous scaffolds were investigated by mechanical and swelling tests, and effect on the morphology of scaffolds examined microscopically. The biodegradation and cytocompatibility tests were used to investigate the effects of zinc oxide nanoparticles incorporation on the ability of scaffolds to use for tissue engineering application. The mean pore size and swelling ratio of scaffolds were decreased upon incorporation of zinc oxide nanoparticles however, the porosity, tensile modulus and biodegradation rate were increased upon incorporation of zinc oxide nanoparticles. In vitro culture of human fibroblasts and keratinocytes showed that the zinc oxide nanoparticles facilitated cell adhesion, proliferation and infiltration of chitosan-collagen 3D porous scaffolds. It was found that the zinc oxide nanoparticles incorporation enhanced porosity, tensile modulus and cytocompatibility of chitosan-collagen 3D porous scaffolds.",
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AU - Idrus, Ruszymah

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