Incorporation of silver nanoparticles (AgNPs) with endophytes cell-free secondary metabolites enhances antimicrobial activity

Ahmad Syairazie Ibrahim Fikri, Irman Abdul Rahman, Ainon Hamzah, Siti Noor Adnalizawati Adnan, Norefrina Shafinaz Md Nor

Research output: Contribution to journalArticle

Abstract

In our previous study, we isolated eight bacterial endophytes with positive antimicrobial activity out of 82 isolates from five different places in Pulau Langkawi. The antimicrobial activity of the endophytes secondary metabolite although moderate, was further explored for the prospect to be enhanced by incorporating to silver nitrates. Only two isolates were able to synthesise silver nanoparticles namely Bacillus subtilis (B7b) and Pseudomonas resinovorans (B2c) within 72 hours of incubation at room temperature via extracellular method. Antimicrobial assay against Staphylococcus aureus, methicilin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermis, Escherichia coli and Pseudomonas aeruginosa was performed. Synthesised silver nanoparticles (AgNPs) showed excellent inhibitory effect against both Gram positive and Gram negative bacteria but secondary metabolites without AgNPs showed positive activity only against Gram positive bacteria. The results suggested that incorporation of AgNPs was able to enhance antimicrobial activity of secondary metabolites of B7b and B2c against test bacteria.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalMalaysian Applied Biology
Volume47
Issue number5
Publication statusPublished - 1 Nov 2018

Fingerprint

nanosilver
Endophytes
endophytes
Silver
Nanoparticles
secondary metabolites
Staphylococcus aureus
anti-infective agents
Silver Nitrate
Gram-Positive Bacteria
Pseudomonas
Bacillus subtilis
Gram-Negative Bacteria
Staphylococcus
Epidermis
Pseudomonas resinovorans
Pseudomonas aeruginosa
cells
Escherichia coli
Bacteria

Keywords

  • Antibacterial
  • Biosynthesis
  • Multidrug-resistant bacteria (MDR)
  • Silver nanoparticles (AgNPs)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Incorporation of silver nanoparticles (AgNPs) with endophytes cell-free secondary metabolites enhances antimicrobial activity. / Fikri, Ahmad Syairazie Ibrahim; Abdul Rahman, Irman; Hamzah, Ainon; Adnan, Siti Noor Adnalizawati; Md Nor, Norefrina Shafinaz.

In: Malaysian Applied Biology, Vol. 47, No. 5, 01.11.2018, p. 283-288.

Research output: Contribution to journalArticle

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