Incorporation of CO2 during the production of succinic acid from sustainable oil palm frond juice

Jian Ping Tan, Abdullah Amru Indera Luthfi, Shareena Fairuz Abdul Manaf, Ta Yeong Wu, Jamaliah Md Jahim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The utilization of the juice from the cheapest carbon source oil palm frond (22 $/ton) in a 3.5 L bioreactor was proven to be competent in replacing pure sugar in succinic acid production. The current study provides an in-depth discussion on the utilization of CO2 in the production of succinic acid. In the serum-bottle fermentation, the yield of succinic acid increased by 51% from 0.47 to 0.71 g/g while the final titer of succinic acid enhanced by 4 fold were attributed to the anaplerotic reaction of Actinobacillus succinogenes and the alkalinity effect of the dissolved CO2. In the bioreactor, the highest mass transfer in the fermentation medium was achieved at 0.5 vvm CO2 at which 3 M KOH was pumped in at a rate of 2.0 mL/min to neutralize the carbonic acid formation, which afforded the highest succinic acid concentration of 30.7 g/L. A first report on actual stoichiometry equation relating substrate, CO2 consumption and all products formation was derived from the results of bioreactor fermentation to lay as a guideline for future reference of actual succinic acid production reaction. C6H12O6 + 0.145 NH3 + 0.642 CO2 → 1.083 C4H6O4 + 0.509 CH2O2 + 0.420 C2H4O2 + 0.117 C2H5OH + 0.726 CH1.8N0.2O0.5 + 0.614 H2O. Actual stoichiometry equation revealed that for every kg of succinic acid produced, 0.22 kg of CO2 will be absorbed. The desirable effect of CO2 on the production of succinic acid may translate into a mutually-beneficial relationship between the succinic acid and the CO2-generating biofuel industries.

Original languageEnglish
Pages (from-to)595-601
Number of pages7
JournalJournal of CO2 Utilization
Volume26
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Palm oil
Succinic Acid
Acids
oil
acid
Bioreactors
Fermentation
bioreactor
fermentation
Stoichiometry
stoichiometry
Carbonic Acid
incorporation
Biofuels
Bottles
Alkalinity
Sugars
biofuel
alkalinity
Carbon

Keywords

  • Actual stoichiometry
  • Biochemical
  • Fine chemical
  • Oil palm frond
  • Succinic acid

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Waste Management and Disposal
  • Process Chemistry and Technology

Cite this

Incorporation of CO2 during the production of succinic acid from sustainable oil palm frond juice. / Tan, Jian Ping; Luthfi, Abdullah Amru Indera; Manaf, Shareena Fairuz Abdul; Wu, Ta Yeong; Md Jahim, Jamaliah.

In: Journal of CO2 Utilization, Vol. 26, 01.07.2018, p. 595-601.

Research output: Contribution to journalArticle

Tan, Jian Ping ; Luthfi, Abdullah Amru Indera ; Manaf, Shareena Fairuz Abdul ; Wu, Ta Yeong ; Md Jahim, Jamaliah. / Incorporation of CO2 during the production of succinic acid from sustainable oil palm frond juice. In: Journal of CO2 Utilization. 2018 ; Vol. 26. pp. 595-601.
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AU - Wu, Ta Yeong

AU - Md Jahim, Jamaliah

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AB - The utilization of the juice from the cheapest carbon source oil palm frond (22 $/ton) in a 3.5 L bioreactor was proven to be competent in replacing pure sugar in succinic acid production. The current study provides an in-depth discussion on the utilization of CO2 in the production of succinic acid. In the serum-bottle fermentation, the yield of succinic acid increased by 51% from 0.47 to 0.71 g/g while the final titer of succinic acid enhanced by 4 fold were attributed to the anaplerotic reaction of Actinobacillus succinogenes and the alkalinity effect of the dissolved CO2. In the bioreactor, the highest mass transfer in the fermentation medium was achieved at 0.5 vvm CO2 at which 3 M KOH was pumped in at a rate of 2.0 mL/min to neutralize the carbonic acid formation, which afforded the highest succinic acid concentration of 30.7 g/L. A first report on actual stoichiometry equation relating substrate, CO2 consumption and all products formation was derived from the results of bioreactor fermentation to lay as a guideline for future reference of actual succinic acid production reaction. C6H12O6 + 0.145 NH3 + 0.642 CO2 → 1.083 C4H6O4 + 0.509 CH2O2 + 0.420 C2H4O2 + 0.117 C2H5OH + 0.726 CH1.8N0.2O0.5 + 0.614 H2O. Actual stoichiometry equation revealed that for every kg of succinic acid produced, 0.22 kg of CO2 will be absorbed. The desirable effect of CO2 on the production of succinic acid may translate into a mutually-beneficial relationship between the succinic acid and the CO2-generating biofuel industries.

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