Incidence of red cell alloantibody among the transfusion recipients of Universiti Kebangsaan Malaysia medical centre

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Red blood cell alloimmunization is a common complication among the transfusion recipients. In Malaysia, multiple ethnicity causes genetic heterogeneity among the population which in turn can cause a wide variation of antibody. The objective of this study was to analyse the red cell alloantibody detected during the pre-transfusion testing. This was a cross-sectional study done in the blood bank of Universiti Kebangsaan Malaysia Medical Centre during the period of January-December 2010. The data was retrieved from the hospital laboratory information system. A total of 24,263 patients' blood samples were subjected for pre-transfusion testing. Antibody screening was done using an indirect antiglobulin test method. The positive samples were further identified for antibody specificity. Antibody screening tests were positive in 184 patients out of 24,263 samples with the incidence of 0.76 %. Autoantibodies and alloantibodies were detected in 39/184 (21.2 %) and 140/184 (76.1 %) of the patients respectively. In five patients (2.7 %) the antibody specificity remained undetermined. Total 161 alloantibodies were identified. The suspected Anti-Mia alloantibody was observed most frequently (49/161, 30.4 %) followed by anti-E (30/161, 18.6 %) and anti-D (22/161, 13.7 %). Anti-E and anti-c were the most common combination of multiple alloantibodies. In view of the high incidence of suspected Anti-Mia antibodies, more efforts are needed to look into the techniques for confirmation of the Anti-Mia antibodies. Besides that, we suggested that all multiply transfused patients should be phenotyped for the Rh system and to supply Rh phenotype specific blood in order to limit alloimmunization.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalIndian Journal of Hematology and Blood Transfusion
Volume29
Issue number2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Isoantibodies
Malaysia
Incidence
Antibody Specificity
Antibodies
Anti-Idiotypic Antibodies
Clinical Laboratory Information Systems
Hospital Information Systems
Coombs Test
Blood Banks
Hospital Laboratories
Genetic Heterogeneity
Autoantibodies
Cross-Sectional Studies
Erythrocytes
Phenotype
Population

Keywords

  • Alloimmunization
  • Antibody specificity
  • Red cell antibody
  • Transfusion recipient

ASJC Scopus subject areas

  • Hematology

Cite this

@article{875ea8f44b234cbab78d2c5cdae0b9cd,
title = "Incidence of red cell alloantibody among the transfusion recipients of Universiti Kebangsaan Malaysia medical centre",
abstract = "Red blood cell alloimmunization is a common complication among the transfusion recipients. In Malaysia, multiple ethnicity causes genetic heterogeneity among the population which in turn can cause a wide variation of antibody. The objective of this study was to analyse the red cell alloantibody detected during the pre-transfusion testing. This was a cross-sectional study done in the blood bank of Universiti Kebangsaan Malaysia Medical Centre during the period of January-December 2010. The data was retrieved from the hospital laboratory information system. A total of 24,263 patients' blood samples were subjected for pre-transfusion testing. Antibody screening was done using an indirect antiglobulin test method. The positive samples were further identified for antibody specificity. Antibody screening tests were positive in 184 patients out of 24,263 samples with the incidence of 0.76 {\%}. Autoantibodies and alloantibodies were detected in 39/184 (21.2 {\%}) and 140/184 (76.1 {\%}) of the patients respectively. In five patients (2.7 {\%}) the antibody specificity remained undetermined. Total 161 alloantibodies were identified. The suspected Anti-Mia alloantibody was observed most frequently (49/161, 30.4 {\%}) followed by anti-E (30/161, 18.6 {\%}) and anti-D (22/161, 13.7 {\%}). Anti-E and anti-c were the most common combination of multiple alloantibodies. In view of the high incidence of suspected Anti-Mia antibodies, more efforts are needed to look into the techniques for confirmation of the Anti-Mia antibodies. Besides that, we suggested that all multiply transfused patients should be phenotyped for the Rh system and to supply Rh phenotype specific blood in order to limit alloimmunization.",
keywords = "Alloimmunization, Antibody specificity, Red cell antibody, Transfusion recipient",
author = "Rabeya Yousuf and {Abdul Aziz}, Suria and Nurasyikin Yusof and {Chooi Fun}, Leong",
year = "2013",
month = "6",
doi = "10.1007/s12288-012-0155-x",
language = "English",
volume = "29",
pages = "65--70",
journal = "Indian Journal of Hematology and Blood Transfusion",
issn = "0971-4502",
publisher = "Springer India",
number = "2",

}

TY - JOUR

T1 - Incidence of red cell alloantibody among the transfusion recipients of Universiti Kebangsaan Malaysia medical centre

AU - Yousuf, Rabeya

AU - Abdul Aziz, Suria

AU - Yusof, Nurasyikin

AU - Chooi Fun, Leong

PY - 2013/6

Y1 - 2013/6

N2 - Red blood cell alloimmunization is a common complication among the transfusion recipients. In Malaysia, multiple ethnicity causes genetic heterogeneity among the population which in turn can cause a wide variation of antibody. The objective of this study was to analyse the red cell alloantibody detected during the pre-transfusion testing. This was a cross-sectional study done in the blood bank of Universiti Kebangsaan Malaysia Medical Centre during the period of January-December 2010. The data was retrieved from the hospital laboratory information system. A total of 24,263 patients' blood samples were subjected for pre-transfusion testing. Antibody screening was done using an indirect antiglobulin test method. The positive samples were further identified for antibody specificity. Antibody screening tests were positive in 184 patients out of 24,263 samples with the incidence of 0.76 %. Autoantibodies and alloantibodies were detected in 39/184 (21.2 %) and 140/184 (76.1 %) of the patients respectively. In five patients (2.7 %) the antibody specificity remained undetermined. Total 161 alloantibodies were identified. The suspected Anti-Mia alloantibody was observed most frequently (49/161, 30.4 %) followed by anti-E (30/161, 18.6 %) and anti-D (22/161, 13.7 %). Anti-E and anti-c were the most common combination of multiple alloantibodies. In view of the high incidence of suspected Anti-Mia antibodies, more efforts are needed to look into the techniques for confirmation of the Anti-Mia antibodies. Besides that, we suggested that all multiply transfused patients should be phenotyped for the Rh system and to supply Rh phenotype specific blood in order to limit alloimmunization.

AB - Red blood cell alloimmunization is a common complication among the transfusion recipients. In Malaysia, multiple ethnicity causes genetic heterogeneity among the population which in turn can cause a wide variation of antibody. The objective of this study was to analyse the red cell alloantibody detected during the pre-transfusion testing. This was a cross-sectional study done in the blood bank of Universiti Kebangsaan Malaysia Medical Centre during the period of January-December 2010. The data was retrieved from the hospital laboratory information system. A total of 24,263 patients' blood samples were subjected for pre-transfusion testing. Antibody screening was done using an indirect antiglobulin test method. The positive samples were further identified for antibody specificity. Antibody screening tests were positive in 184 patients out of 24,263 samples with the incidence of 0.76 %. Autoantibodies and alloantibodies were detected in 39/184 (21.2 %) and 140/184 (76.1 %) of the patients respectively. In five patients (2.7 %) the antibody specificity remained undetermined. Total 161 alloantibodies were identified. The suspected Anti-Mia alloantibody was observed most frequently (49/161, 30.4 %) followed by anti-E (30/161, 18.6 %) and anti-D (22/161, 13.7 %). Anti-E and anti-c were the most common combination of multiple alloantibodies. In view of the high incidence of suspected Anti-Mia antibodies, more efforts are needed to look into the techniques for confirmation of the Anti-Mia antibodies. Besides that, we suggested that all multiply transfused patients should be phenotyped for the Rh system and to supply Rh phenotype specific blood in order to limit alloimmunization.

KW - Alloimmunization

KW - Antibody specificity

KW - Red cell antibody

KW - Transfusion recipient

UR - http://www.scopus.com/inward/record.url?scp=84882814313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882814313&partnerID=8YFLogxK

U2 - 10.1007/s12288-012-0155-x

DO - 10.1007/s12288-012-0155-x

M3 - Article

AN - SCOPUS:84882814313

VL - 29

SP - 65

EP - 70

JO - Indian Journal of Hematology and Blood Transfusion

JF - Indian Journal of Hematology and Blood Transfusion

SN - 0971-4502

IS - 2

ER -