Inactivation of the catalytic subunit of cAMP-dependent protein Kinase A causes delayed appressorium formation and reduced pathogenicity of Colletotrichum gloeosporioides

Tri Puji Priyatno, Farah Diba Abu Bakar, Nurhaida Kamaruddin, Nor Muhammad Mahadi, Abdul Munir Abd. Murad

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13 Citations (Scopus)

Abstract

The cyclic AMP- (cAMP-) dependent protein kinase A signaling pathway is one of the major signaling pathways responsible for regulation of the morphogenesis and pathogenesis of several pathogenic fungi. To evaluate the role of this pathway in the plant pathogenic fungus, Colletotrichum gloeosporioides, the gene encoding the catalytic subunit of cAMP-dependent protein kinase A, CgPKAC, was cloned, inactivated, and the mutant was analyzed. Analysis of the Cgpkac mutant generated via gene replacement showed that the mutants were able to form appressoria; however, their formation was delayed compared to the wild type. In addition, the mutant conidia underwent bipolar germination after appressoria formation, but no appressoria were generated from the second germ tube. The mutants also showed reduced ability to adhere to a hydrophobic surface and to degrade lipids localized in the appressoria. Based on the number of lesions produced during a pathogenicity test, the mutant's ability to cause disease in healthy mango fruits was reduced, which may be due to failure to penetrate into the fruit. These findings indicate that cAMP-dependent protein kinase A has an important role in regulating morphogenesis and is required for pathogenicity of C. gloeosporioides.

Original languageEnglish
Article number545784
JournalThe Scientific World Journal
Volume2012
DOIs
Publication statusPublished - 2012

Fingerprint

Colletotrichum
pathogenicity
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
Virulence
Catalytic Domain
protein
Fruits
Fungi
Morphogenesis
morphogenesis
Fruit
Mangifera
fruit
fungus
Gene encoding
Fungal Spores
Germination
gene
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

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title = "Inactivation of the catalytic subunit of cAMP-dependent protein Kinase A causes delayed appressorium formation and reduced pathogenicity of Colletotrichum gloeosporioides",
abstract = "The cyclic AMP- (cAMP-) dependent protein kinase A signaling pathway is one of the major signaling pathways responsible for regulation of the morphogenesis and pathogenesis of several pathogenic fungi. To evaluate the role of this pathway in the plant pathogenic fungus, Colletotrichum gloeosporioides, the gene encoding the catalytic subunit of cAMP-dependent protein kinase A, CgPKAC, was cloned, inactivated, and the mutant was analyzed. Analysis of the Cgpkac mutant generated via gene replacement showed that the mutants were able to form appressoria; however, their formation was delayed compared to the wild type. In addition, the mutant conidia underwent bipolar germination after appressoria formation, but no appressoria were generated from the second germ tube. The mutants also showed reduced ability to adhere to a hydrophobic surface and to degrade lipids localized in the appressoria. Based on the number of lesions produced during a pathogenicity test, the mutant's ability to cause disease in healthy mango fruits was reduced, which may be due to failure to penetrate into the fruit. These findings indicate that cAMP-dependent protein kinase A has an important role in regulating morphogenesis and is required for pathogenicity of C. gloeosporioides.",
author = "Priyatno, {Tri Puji} and {Abu Bakar}, {Farah Diba} and Nurhaida Kamaruddin and Mahadi, {Nor Muhammad} and {Abd. Murad}, {Abdul Munir}",
year = "2012",
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AU - Priyatno, Tri Puji

AU - Abu Bakar, Farah Diba

AU - Kamaruddin, Nurhaida

AU - Mahadi, Nor Muhammad

AU - Abd. Murad, Abdul Munir

PY - 2012

Y1 - 2012

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AB - The cyclic AMP- (cAMP-) dependent protein kinase A signaling pathway is one of the major signaling pathways responsible for regulation of the morphogenesis and pathogenesis of several pathogenic fungi. To evaluate the role of this pathway in the plant pathogenic fungus, Colletotrichum gloeosporioides, the gene encoding the catalytic subunit of cAMP-dependent protein kinase A, CgPKAC, was cloned, inactivated, and the mutant was analyzed. Analysis of the Cgpkac mutant generated via gene replacement showed that the mutants were able to form appressoria; however, their formation was delayed compared to the wild type. In addition, the mutant conidia underwent bipolar germination after appressoria formation, but no appressoria were generated from the second germ tube. The mutants also showed reduced ability to adhere to a hydrophobic surface and to degrade lipids localized in the appressoria. Based on the number of lesions produced during a pathogenicity test, the mutant's ability to cause disease in healthy mango fruits was reduced, which may be due to failure to penetrate into the fruit. These findings indicate that cAMP-dependent protein kinase A has an important role in regulating morphogenesis and is required for pathogenicity of C. gloeosporioides.

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