In vivo response of GsdmA3Dfl/+ mice to topically applied fish oil – effects on cellular markers and macrophages

Mohd Hanif Zulfakar, Rebecca M. Porter, Charles M. Heard

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Psoriasis is an incurable autoimmune disease characterized by patches of abnormal red, itchy and scaly skin. This work examined the modulation of inflammation, hyperproliferation and immune cell markers following topical application of fish oil (FO) in comparison to the antipsoriatic agents, betamethasone dipropionate (BD) and salicylic acid (SA), to GsdmA3Dfl/+ mice, a hair loss mutant which also exhibits epidermal hyperproliferation akin to psoriasis. The mice were dosed with 100 mg of the test formulation and after 10 days, the mice were sacrificed, skin sections excised and subjected to immunohistochemical determination of COX-2, K17 and MAC-1; and immunofluorescence of Ki-67. Unchanged expression of the proinflammatory enzyme COX-2 was observed in all treatments, suggesting the non-involvement of COX-2 in the aetiology of cutaneous aberration seen in GsdmA3Dfl/+ mice. Intense staining of K17 and MAC-1 in the FO-treated group mirrored the epidermal thickening seen observed in live mice by optical coherence tomography (OCT). The ratio of Ki-67-positive nuclei per 100 basal cells indicated that hyperproliferation of keratinocytes occurred in FO-treated mice and the opposite was true for BD-treated mice. There was a positive correlation (R2 0.995) between Ki-67 and the epidermal thickness data observed previously. In all immunochemical procedures, the combined BD, SA and FO formulation did not show any significant difference with the control group, reflecting observations seen previously. In conclusion, the epidermal changes observed following topical FO treatment on GsdmA3Dfl/+ mice involves an increase in cellular proliferation and macrophages, although COX-2 does not appear to play an important role.

Original languageEnglish
Pages (from-to)827-834
Number of pages8
JournalFEBS Open Bio
Volume6
Issue number8
DOIs
Publication statusPublished - 1 Jan 2016

Fingerprint

Fish Oils
Macrophages
Skin
Psoriasis
Optical tomography
Aberrations
Cells
Modulation
Alopecia
Optical Coherence Tomography
Keratinocytes
Autoimmune Diseases
Fluorescent Antibody Technique
Enzymes
Biomarkers
Cell Proliferation
Staining and Labeling
Inflammation
Control Groups
Therapeutics

Keywords

  • Cellular markers
  • Fish oil
  • Mouse
  • Omega 3 fatty acids
  • Skin
  • Topical delivery

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

In vivo response of GsdmA3Dfl/+ mice to topically applied fish oil – effects on cellular markers and macrophages. / Zulfakar, Mohd Hanif; Porter, Rebecca M.; Heard, Charles M.

In: FEBS Open Bio, Vol. 6, No. 8, 01.01.2016, p. 827-834.

Research output: Contribution to journalArticle

@article{371654fcc18242b99cbb2993b504ed89,
title = "In vivo response of GsdmA3Dfl/+ mice to topically applied fish oil – effects on cellular markers and macrophages",
abstract = "Psoriasis is an incurable autoimmune disease characterized by patches of abnormal red, itchy and scaly skin. This work examined the modulation of inflammation, hyperproliferation and immune cell markers following topical application of fish oil (FO) in comparison to the antipsoriatic agents, betamethasone dipropionate (BD) and salicylic acid (SA), to GsdmA3Dfl/+ mice, a hair loss mutant which also exhibits epidermal hyperproliferation akin to psoriasis. The mice were dosed with 100 mg of the test formulation and after 10 days, the mice were sacrificed, skin sections excised and subjected to immunohistochemical determination of COX-2, K17 and MAC-1; and immunofluorescence of Ki-67. Unchanged expression of the proinflammatory enzyme COX-2 was observed in all treatments, suggesting the non-involvement of COX-2 in the aetiology of cutaneous aberration seen in GsdmA3Dfl/+ mice. Intense staining of K17 and MAC-1 in the FO-treated group mirrored the epidermal thickening seen observed in live mice by optical coherence tomography (OCT). The ratio of Ki-67-positive nuclei per 100 basal cells indicated that hyperproliferation of keratinocytes occurred in FO-treated mice and the opposite was true for BD-treated mice. There was a positive correlation (R2 0.995) between Ki-67 and the epidermal thickness data observed previously. In all immunochemical procedures, the combined BD, SA and FO formulation did not show any significant difference with the control group, reflecting observations seen previously. In conclusion, the epidermal changes observed following topical FO treatment on GsdmA3Dfl/+ mice involves an increase in cellular proliferation and macrophages, although COX-2 does not appear to play an important role.",
keywords = "Cellular markers, Fish oil, Mouse, Omega 3 fatty acids, Skin, Topical delivery",
author = "Zulfakar, {Mohd Hanif} and Porter, {Rebecca M.} and Heard, {Charles M.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/2211-5463.12095",
language = "English",
volume = "6",
pages = "827--834",
journal = "FEBS Open Bio",
issn = "2211-5463",
publisher = "Elsevier BV",
number = "8",

}

TY - JOUR

T1 - In vivo response of GsdmA3Dfl/+ mice to topically applied fish oil – effects on cellular markers and macrophages

AU - Zulfakar, Mohd Hanif

AU - Porter, Rebecca M.

AU - Heard, Charles M.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Psoriasis is an incurable autoimmune disease characterized by patches of abnormal red, itchy and scaly skin. This work examined the modulation of inflammation, hyperproliferation and immune cell markers following topical application of fish oil (FO) in comparison to the antipsoriatic agents, betamethasone dipropionate (BD) and salicylic acid (SA), to GsdmA3Dfl/+ mice, a hair loss mutant which also exhibits epidermal hyperproliferation akin to psoriasis. The mice were dosed with 100 mg of the test formulation and after 10 days, the mice were sacrificed, skin sections excised and subjected to immunohistochemical determination of COX-2, K17 and MAC-1; and immunofluorescence of Ki-67. Unchanged expression of the proinflammatory enzyme COX-2 was observed in all treatments, suggesting the non-involvement of COX-2 in the aetiology of cutaneous aberration seen in GsdmA3Dfl/+ mice. Intense staining of K17 and MAC-1 in the FO-treated group mirrored the epidermal thickening seen observed in live mice by optical coherence tomography (OCT). The ratio of Ki-67-positive nuclei per 100 basal cells indicated that hyperproliferation of keratinocytes occurred in FO-treated mice and the opposite was true for BD-treated mice. There was a positive correlation (R2 0.995) between Ki-67 and the epidermal thickness data observed previously. In all immunochemical procedures, the combined BD, SA and FO formulation did not show any significant difference with the control group, reflecting observations seen previously. In conclusion, the epidermal changes observed following topical FO treatment on GsdmA3Dfl/+ mice involves an increase in cellular proliferation and macrophages, although COX-2 does not appear to play an important role.

AB - Psoriasis is an incurable autoimmune disease characterized by patches of abnormal red, itchy and scaly skin. This work examined the modulation of inflammation, hyperproliferation and immune cell markers following topical application of fish oil (FO) in comparison to the antipsoriatic agents, betamethasone dipropionate (BD) and salicylic acid (SA), to GsdmA3Dfl/+ mice, a hair loss mutant which also exhibits epidermal hyperproliferation akin to psoriasis. The mice were dosed with 100 mg of the test formulation and after 10 days, the mice were sacrificed, skin sections excised and subjected to immunohistochemical determination of COX-2, K17 and MAC-1; and immunofluorescence of Ki-67. Unchanged expression of the proinflammatory enzyme COX-2 was observed in all treatments, suggesting the non-involvement of COX-2 in the aetiology of cutaneous aberration seen in GsdmA3Dfl/+ mice. Intense staining of K17 and MAC-1 in the FO-treated group mirrored the epidermal thickening seen observed in live mice by optical coherence tomography (OCT). The ratio of Ki-67-positive nuclei per 100 basal cells indicated that hyperproliferation of keratinocytes occurred in FO-treated mice and the opposite was true for BD-treated mice. There was a positive correlation (R2 0.995) between Ki-67 and the epidermal thickness data observed previously. In all immunochemical procedures, the combined BD, SA and FO formulation did not show any significant difference with the control group, reflecting observations seen previously. In conclusion, the epidermal changes observed following topical FO treatment on GsdmA3Dfl/+ mice involves an increase in cellular proliferation and macrophages, although COX-2 does not appear to play an important role.

KW - Cellular markers

KW - Fish oil

KW - Mouse

KW - Omega 3 fatty acids

KW - Skin

KW - Topical delivery

UR - http://www.scopus.com/inward/record.url?scp=85055468543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055468543&partnerID=8YFLogxK

U2 - 10.1002/2211-5463.12095

DO - 10.1002/2211-5463.12095

M3 - Article

VL - 6

SP - 827

EP - 834

JO - FEBS Open Bio

JF - FEBS Open Bio

SN - 2211-5463

IS - 8

ER -