In vivo response of GsdmA3Dfl/+ mice to topically applied anti-psoriatic agents

Effects on epidermal thickness, as determined by optical coherence tomography and H&E staining

Mohd Hanif Zulfakar, Aneesh Alex, Boris Povazay, Wolfgang Drexler, Christopher P. Thomas, Rebecca M. Porter, Charles M. Heard

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study evaluated in vivo the potential of optical coherence tomography (OCT) to determine changes in thickness of the epidermis in response to the topically applied anti-psoriatics betamethasone dipropionate (BD), salicylic acid (SA) and also fish oil (FO). GsdmA3Dfl/+ mice have an inflammatory hair loss phenotype that includes hyperproliferation and epidermal thickening, hence a potential psoriasis model. Changes in epidermal thickness were evaluated over a period of 10days, with the mice treated with combined BD+SA, FO+SA and BD+FO+SA. The data were validated with conventional measurement using H&E staining coupled with microscopy. Initial baseline measurement revealed an average epidermal thickness of 26.92±1.17μm. After 10days of treatment with BD, the average epidermal thickness was reduced by 38.8% (P=0.0001), and inversely, treatment with FO resulted in an unexpected 105% increase (P=0.0001) in epidermal thickness. Combined BD+FO treatment did not cause any significant change (P=0.3755) and may further indicate opposing effects on keratinocyte proliferation. The data obtained using OCT were statistically the same as those obtained by H&E/microscopy (P=0.4325), supporting a greater role for OCT in dermatological studies, while also allowing a reduction in the number of animals used in such studies as sacrifice at individual timepoints is not necessary.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalExperimental Dermatology
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Fish Oils
Optical tomography
Optical Coherence Tomography
Staining and Labeling
Microscopy
Microscopic examination
Salicylic Acid
Alopecia
Keratinocytes
Psoriasis
Epidermis
Animals
Phenotype
salicylic acid drug combination betamethasone dipropionate
betamethasone-17,21-dipropionate

Keywords

  • Betamethasone
  • Fish oil
  • Mouse model
  • Optical coherence tomography
  • Psoriasis

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

In vivo response of GsdmA3Dfl/+ mice to topically applied anti-psoriatic agents : Effects on epidermal thickness, as determined by optical coherence tomography and H&E staining. / Zulfakar, Mohd Hanif; Alex, Aneesh; Povazay, Boris; Drexler, Wolfgang; Thomas, Christopher P.; Porter, Rebecca M.; Heard, Charles M.

In: Experimental Dermatology, Vol. 20, No. 3, 03.2011, p. 269-272.

Research output: Contribution to journalArticle

Zulfakar, Mohd Hanif ; Alex, Aneesh ; Povazay, Boris ; Drexler, Wolfgang ; Thomas, Christopher P. ; Porter, Rebecca M. ; Heard, Charles M. / In vivo response of GsdmA3Dfl/+ mice to topically applied anti-psoriatic agents : Effects on epidermal thickness, as determined by optical coherence tomography and H&E staining. In: Experimental Dermatology. 2011 ; Vol. 20, No. 3. pp. 269-272.
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