In vivo production of Nosema bombycis spores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions

Syarafina Ramli, B. A H Zainal-Abidin, Idris Abd. Ghani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome this problem. Nosema bombycis is a microsporidial pathogen with the potential to control insect pests. This study was aimed at producing N. bombycis spores in vivo and to test their efficacies against DBM and BAW in laboratory conditions. Production of this pathogen was carried out by feeding both DBM and BAW larval instars with artificial diet inoculated with viable spores of N. bombycis. Spore concentrations in the larvae were determined and accumulated. If a concentration of 1.56 × 108 spores/mL was obtained, the production was continued. For laboratory efficacy tests, artificial diets with different spore concentrations namely 1 × l04, 1 × 105 and 1 × 106 spores/μL were fed to different larval instar. Mortality rates were analysed using the Two-way ANOVA test. For both DBM and BAW, third instar showed the highest mean mortality rates and the mortality in each instars were significantly different (P<0.05). The concentration 1 × 106 spores/μL caused the highest mean mortality rates and each concentration resulted in significantly different mortality (P<0.05). However, there was no interaction between both factors, where a combination of spore concentrations and instars did not show any significant differences ( P>0.05). These results indicated that third instar larvae for both species and 1 × 106 spores/μL treatments demonstrated the highest efficacy rates.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalSains Malaysiana
Volume40
Issue number4
Publication statusPublished - Apr 2011

Fingerprint

Nosema bombycis
Spodoptera exigua
Plutella xylostella
spores
larvae
instars
artificial diets
insect pests
biopesticides
pathogens
testing
biological control agents
insecticides
analysis of variance
pests

Keywords

  • Nosema bombycis
  • Plutella xylostella
  • Spodoptera exigua

ASJC Scopus subject areas

  • General

Cite this

In vivo production of Nosema bombycis spores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions. / Ramli, Syarafina; Zainal-Abidin, B. A H; Abd. Ghani, Idris.

In: Sains Malaysiana, Vol. 40, No. 4, 04.2011, p. 311-316.

Research output: Contribution to journalArticle

Ramli, Syarafina ; Zainal-Abidin, B. A H ; Abd. Ghani, Idris. / In vivo production of Nosema bombycis spores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions. In: Sains Malaysiana. 2011 ; Vol. 40, No. 4. pp. 311-316.
@article{91b44485b44a4846b7539bb1ec3d22cb,
title = "In vivo production of Nosema bombycis spores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions",
abstract = "Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome this problem. Nosema bombycis is a microsporidial pathogen with the potential to control insect pests. This study was aimed at producing N. bombycis spores in vivo and to test their efficacies against DBM and BAW in laboratory conditions. Production of this pathogen was carried out by feeding both DBM and BAW larval instars with artificial diet inoculated with viable spores of N. bombycis. Spore concentrations in the larvae were determined and accumulated. If a concentration of 1.56 × 108 spores/mL was obtained, the production was continued. For laboratory efficacy tests, artificial diets with different spore concentrations namely 1 × l04, 1 × 105 and 1 × 106 spores/μL were fed to different larval instar. Mortality rates were analysed using the Two-way ANOVA test. For both DBM and BAW, third instar showed the highest mean mortality rates and the mortality in each instars were significantly different (P<0.05). The concentration 1 × 106 spores/μL caused the highest mean mortality rates and each concentration resulted in significantly different mortality (P<0.05). However, there was no interaction between both factors, where a combination of spore concentrations and instars did not show any significant differences ( P>0.05). These results indicated that third instar larvae for both species and 1 × 106 spores/μL treatments demonstrated the highest efficacy rates.",
keywords = "Nosema bombycis, Plutella xylostella, Spodoptera exigua",
author = "Syarafina Ramli and Zainal-Abidin, {B. A H} and {Abd. Ghani}, Idris",
year = "2011",
month = "4",
language = "English",
volume = "40",
pages = "311--316",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "4",

}

TY - JOUR

T1 - In vivo production of Nosema bombycis spores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions

AU - Ramli, Syarafina

AU - Zainal-Abidin, B. A H

AU - Abd. Ghani, Idris

PY - 2011/4

Y1 - 2011/4

N2 - Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome this problem. Nosema bombycis is a microsporidial pathogen with the potential to control insect pests. This study was aimed at producing N. bombycis spores in vivo and to test their efficacies against DBM and BAW in laboratory conditions. Production of this pathogen was carried out by feeding both DBM and BAW larval instars with artificial diet inoculated with viable spores of N. bombycis. Spore concentrations in the larvae were determined and accumulated. If a concentration of 1.56 × 108 spores/mL was obtained, the production was continued. For laboratory efficacy tests, artificial diets with different spore concentrations namely 1 × l04, 1 × 105 and 1 × 106 spores/μL were fed to different larval instar. Mortality rates were analysed using the Two-way ANOVA test. For both DBM and BAW, third instar showed the highest mean mortality rates and the mortality in each instars were significantly different (P<0.05). The concentration 1 × 106 spores/μL caused the highest mean mortality rates and each concentration resulted in significantly different mortality (P<0.05). However, there was no interaction between both factors, where a combination of spore concentrations and instars did not show any significant differences ( P>0.05). These results indicated that third instar larvae for both species and 1 × 106 spores/μL treatments demonstrated the highest efficacy rates.

AB - Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome this problem. Nosema bombycis is a microsporidial pathogen with the potential to control insect pests. This study was aimed at producing N. bombycis spores in vivo and to test their efficacies against DBM and BAW in laboratory conditions. Production of this pathogen was carried out by feeding both DBM and BAW larval instars with artificial diet inoculated with viable spores of N. bombycis. Spore concentrations in the larvae were determined and accumulated. If a concentration of 1.56 × 108 spores/mL was obtained, the production was continued. For laboratory efficacy tests, artificial diets with different spore concentrations namely 1 × l04, 1 × 105 and 1 × 106 spores/μL were fed to different larval instar. Mortality rates were analysed using the Two-way ANOVA test. For both DBM and BAW, third instar showed the highest mean mortality rates and the mortality in each instars were significantly different (P<0.05). The concentration 1 × 106 spores/μL caused the highest mean mortality rates and each concentration resulted in significantly different mortality (P<0.05). However, there was no interaction between both factors, where a combination of spore concentrations and instars did not show any significant differences ( P>0.05). These results indicated that third instar larvae for both species and 1 × 106 spores/μL treatments demonstrated the highest efficacy rates.

KW - Nosema bombycis

KW - Plutella xylostella

KW - Spodoptera exigua

UR - http://www.scopus.com/inward/record.url?scp=79955585027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955585027&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 311

EP - 316

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 4

ER -