In vivo evaluation of bacterial cellulose/acrylic acid wound dressing hydrogel containing keratinocytes and fibroblasts for burn wounds

Najwa Mohamad, Evelyn Yun Xi Loh, Mh Busra Fauzi, Min Hwei Ng, Mohd Cairul Iqbal Mohd Amin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The healing of wounds, including those from burns, currently exerts a burden on healthcare systems worldwide. Hydrogels are widely used as wound dressings and in the field of tissue engineering. The popularity of bacterial cellulose-based hydrogels has increased owing to their biocompatibility. Previous study demonstrated that bacterial cellulose/acrylic acid (BC/AA) hydrogel increased the healing rate of burn wound. This in vivo study using athymic mice has extended the use of BC/AA hydrogel by the addition of human epidermal keratinocytes and human dermal fibroblasts. The results showed that hydrogel loaded with cells produces the greatest acceleration on burn wound healing, followed by treatment with hydrogel alone, compared with the untreated group. The percentage wound reduction on day 13 in the mice treated with hydrogel loaded with cells (77.34 ± 6.21%) was significantly higher than that in the control-treated mice (64.79 ± 6.84%). Histological analysis, the expression of collagen type I via immunohistochemistry, and transmission electron microscopy indicated a greater deposition of collagen in the mice treated with hydrogel loaded with cells than in the mice administered other treatments. Therefore, the BC/AA hydrogel has promising application as a wound dressing and a cell carrier.

Original languageEnglish
Pages (from-to)444-452
Number of pages9
JournalDrug Delivery and Translational Research
Volume9
Issue number2
DOIs
Publication statusPublished - 15 Apr 2019

Fingerprint

Hydrogel
Bandages
Keratinocytes
Cellulose
Fibroblasts
Wounds and Injuries
Hydrogels
Wound Healing
Tissue Engineering
Collagen Type I
acrylic acid
Transmission Electron Microscopy
Burns
Nude Mice
Collagen
Immunohistochemistry
Delivery of Health Care
Skin
Therapeutics

Keywords

  • Cell carrier
  • Skin injury
  • Tissue regeneration
  • Wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

In vivo evaluation of bacterial cellulose/acrylic acid wound dressing hydrogel containing keratinocytes and fibroblasts for burn wounds. / Mohamad, Najwa; Loh, Evelyn Yun Xi; Fauzi, Mh Busra; Ng, Min Hwei; Mohd Amin, Mohd Cairul Iqbal.

In: Drug Delivery and Translational Research, Vol. 9, No. 2, 15.04.2019, p. 444-452.

Research output: Contribution to journalArticle

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