In vivo effects of stress, ACTH and corticosterone on testicular 11β-hydroxysteroid dehydrogenase oxidative activity in rats and the possible mechanism of actions

K. H H Nwe, Norhazlina Abdul Wahab, A. Hamid, P. B. Morat, B. A K Khalid

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effects of stress and corticosterone on testicular 11β-hydroxysteroid dehydrogenase (11β-HSD) oxidative activity have been controversial, whilst that of adrenocorticotrophic hormone (ACTH) have not been investigated before. Hence, the aim of the present study was to determine the in vivo effects of stress due to injection and sham operation, ACTH and corticosterone on testicular and hepatic 11β-HSD oxidative activity and plasma testosterone levels in normal and adrenalectomized (ADX) rats and their possible mechanism of actions. Adrenalectomy reduced both testicular 11β-HSD oxidative activity and plasma testosterone levels. The effects of injection and sham operation significantly increased plasma corticosterone levels with decreased testicular 11β-HSD oxidative activity and plasma testosterone levels in normal but not in ADX rats. Likewise, ACTH or corticosterone treatment for 7 days decreased both testicular 11β-HSD oxidative activity in a dose dependent manner and plasma testosterone levels in normal rats; but the values in ADX rats remained unchanged. However, none of the above values were significantly lower than that of the ADX levels. Corticosterone seems to maintain testicular 11β-HSD oxidative activity within the range between normal and ADX rats. These changes are not attributable to diurnal rhythms, as the time of sacrifice has been fixed between 8:30 and 10:30 am. In the liver, no significant change in 11β-HSD oxidative activity was observed with sham operation, ACTH or corticosterone treatment; but adrenalectomy significantly decreased it. In conclusion, in the intact normal rats, stress, ACTH or corticosterone modulates testicular (but not hepatic) 11β-HSD oxidative activity indirectly through the adrenal glands and the physiological level of corticosterone is ideal for normal reproductive functions.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalExperimental and Clinical Endocrinology and Diabetes
Volume108
Issue number5
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

11-beta-Hydroxysteroid Dehydrogenases
Corticosterone
Adrenocorticotropic Hormone
Testosterone
Adrenalectomy
Liver
Injections
Adrenal Glands
Circadian Rhythm
Reference Values

Keywords

  • 11β-hydroxysteroid dehydrogenase
  • ACTH
  • Corticosterone
  • Rat
  • Stress
  • Testis
  • Testosterone levels

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

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title = "In vivo effects of stress, ACTH and corticosterone on testicular 11β-hydroxysteroid dehydrogenase oxidative activity in rats and the possible mechanism of actions",
abstract = "The effects of stress and corticosterone on testicular 11β-hydroxysteroid dehydrogenase (11β-HSD) oxidative activity have been controversial, whilst that of adrenocorticotrophic hormone (ACTH) have not been investigated before. Hence, the aim of the present study was to determine the in vivo effects of stress due to injection and sham operation, ACTH and corticosterone on testicular and hepatic 11β-HSD oxidative activity and plasma testosterone levels in normal and adrenalectomized (ADX) rats and their possible mechanism of actions. Adrenalectomy reduced both testicular 11β-HSD oxidative activity and plasma testosterone levels. The effects of injection and sham operation significantly increased plasma corticosterone levels with decreased testicular 11β-HSD oxidative activity and plasma testosterone levels in normal but not in ADX rats. Likewise, ACTH or corticosterone treatment for 7 days decreased both testicular 11β-HSD oxidative activity in a dose dependent manner and plasma testosterone levels in normal rats; but the values in ADX rats remained unchanged. However, none of the above values were significantly lower than that of the ADX levels. Corticosterone seems to maintain testicular 11β-HSD oxidative activity within the range between normal and ADX rats. These changes are not attributable to diurnal rhythms, as the time of sacrifice has been fixed between 8:30 and 10:30 am. In the liver, no significant change in 11β-HSD oxidative activity was observed with sham operation, ACTH or corticosterone treatment; but adrenalectomy significantly decreased it. In conclusion, in the intact normal rats, stress, ACTH or corticosterone modulates testicular (but not hepatic) 11β-HSD oxidative activity indirectly through the adrenal glands and the physiological level of corticosterone is ideal for normal reproductive functions.",
keywords = "11β-hydroxysteroid dehydrogenase, ACTH, Corticosterone, Rat, Stress, Testis, Testosterone levels",
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T1 - In vivo effects of stress, ACTH and corticosterone on testicular 11β-hydroxysteroid dehydrogenase oxidative activity in rats and the possible mechanism of actions

AU - Nwe, K. H H

AU - Abdul Wahab, Norhazlina

AU - Hamid, A.

AU - Morat, P. B.

AU - Khalid, B. A K

PY - 2000

Y1 - 2000

N2 - The effects of stress and corticosterone on testicular 11β-hydroxysteroid dehydrogenase (11β-HSD) oxidative activity have been controversial, whilst that of adrenocorticotrophic hormone (ACTH) have not been investigated before. Hence, the aim of the present study was to determine the in vivo effects of stress due to injection and sham operation, ACTH and corticosterone on testicular and hepatic 11β-HSD oxidative activity and plasma testosterone levels in normal and adrenalectomized (ADX) rats and their possible mechanism of actions. Adrenalectomy reduced both testicular 11β-HSD oxidative activity and plasma testosterone levels. The effects of injection and sham operation significantly increased plasma corticosterone levels with decreased testicular 11β-HSD oxidative activity and plasma testosterone levels in normal but not in ADX rats. Likewise, ACTH or corticosterone treatment for 7 days decreased both testicular 11β-HSD oxidative activity in a dose dependent manner and plasma testosterone levels in normal rats; but the values in ADX rats remained unchanged. However, none of the above values were significantly lower than that of the ADX levels. Corticosterone seems to maintain testicular 11β-HSD oxidative activity within the range between normal and ADX rats. These changes are not attributable to diurnal rhythms, as the time of sacrifice has been fixed between 8:30 and 10:30 am. In the liver, no significant change in 11β-HSD oxidative activity was observed with sham operation, ACTH or corticosterone treatment; but adrenalectomy significantly decreased it. In conclusion, in the intact normal rats, stress, ACTH or corticosterone modulates testicular (but not hepatic) 11β-HSD oxidative activity indirectly through the adrenal glands and the physiological level of corticosterone is ideal for normal reproductive functions.

AB - The effects of stress and corticosterone on testicular 11β-hydroxysteroid dehydrogenase (11β-HSD) oxidative activity have been controversial, whilst that of adrenocorticotrophic hormone (ACTH) have not been investigated before. Hence, the aim of the present study was to determine the in vivo effects of stress due to injection and sham operation, ACTH and corticosterone on testicular and hepatic 11β-HSD oxidative activity and plasma testosterone levels in normal and adrenalectomized (ADX) rats and their possible mechanism of actions. Adrenalectomy reduced both testicular 11β-HSD oxidative activity and plasma testosterone levels. The effects of injection and sham operation significantly increased plasma corticosterone levels with decreased testicular 11β-HSD oxidative activity and plasma testosterone levels in normal but not in ADX rats. Likewise, ACTH or corticosterone treatment for 7 days decreased both testicular 11β-HSD oxidative activity in a dose dependent manner and plasma testosterone levels in normal rats; but the values in ADX rats remained unchanged. However, none of the above values were significantly lower than that of the ADX levels. Corticosterone seems to maintain testicular 11β-HSD oxidative activity within the range between normal and ADX rats. These changes are not attributable to diurnal rhythms, as the time of sacrifice has been fixed between 8:30 and 10:30 am. In the liver, no significant change in 11β-HSD oxidative activity was observed with sham operation, ACTH or corticosterone treatment; but adrenalectomy significantly decreased it. In conclusion, in the intact normal rats, stress, ACTH or corticosterone modulates testicular (but not hepatic) 11β-HSD oxidative activity indirectly through the adrenal glands and the physiological level of corticosterone is ideal for normal reproductive functions.

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KW - Stress

KW - Testis

KW - Testosterone levels

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