In vivo effect of Piper sarmentosum methanolic extract on stress-induced gastric ulcers in rats

Nur Azlina Mohd Fahami, Qodriyah Mohd Saad, Muhamad Nurul Akmal, Ibrahim Abdel Aziz Ibrahim, Kamisah Yusof

Research output: Contribution to journalArticle

Abstract

Introduction: Piper sarmentosum (Piperaceae) is traditionally used by Asians to treat numerous common ailments including asthma, fever and gastritis. The aim of the research was to determine and compare the effects of Piper sarmentosum (PS) with omeprazole (OMZ) on gastric parameters in rats exposed to restraint stress. Material and methods: The methanolic extract of PS was prepared in the dose of 500 mg/kg. Twenty-eight male Wistar rats were assigned to 4 equal sized groups: two control groups and two treated groups which were supplemented with either PS or OMZ orally at a dose of 500 mg/kg and 20 mg/kg body weight respectively. After 28 days of treatment, one control group, the PS and OMZ group were subjected to a single exposure of water-immersion restraint stress for 3.5 h. After the last exposure to stress, the stomach was excised for evaluation of the parameters. Results: Oral supplementation of PS was as effective in preventing the formation of gastric lesion when compared with OMZ (p < 0.05). The increased gastric acidity and MDA due to stress was also reduced with supplementation of PS and OMZ. Only PS had the ability to reduce prostaglandin E2 loss (p = 0.0067) and have the ability to down regulate cyclooxygenase-2 (COX-2) mRNA expression (p = 0.01) with stress exposure. Conclusions: Piper sarmentosum possesses a similar protective effect against stress-induced gastric lesions as omeprazole. The protective effect was associated with decreased lipid peroxidation, increased prostaglandin E2, reduction in gastric acidity and reduction in COX-2 mRNA expression which was altered by stress.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalArchives of Medical Science
Volume15
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Piper
Stomach Ulcer
Omeprazole
Stomach
Cyclooxygenase 2
Dinoprostone
Piperaceae
Control Groups
Messenger RNA
Immersion
Gastritis
Lipid Peroxidation
Wistar Rats
Fever
Down-Regulation
Asthma
Body Weight

Keywords

  • Gastric ulcers
  • Oxidative stress
  • Piper sarmentosum
  • Prostaglandins
  • Stress

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In vivo effect of Piper sarmentosum methanolic extract on stress-induced gastric ulcers in rats. / Mohd Fahami, Nur Azlina; Mohd Saad, Qodriyah; Akmal, Muhamad Nurul; Ibrahim, Ibrahim Abdel Aziz; Yusof, Kamisah.

In: Archives of Medical Science, Vol. 15, No. 1, 01.01.2019, p. 223-231.

Research output: Contribution to journalArticle

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