In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice

Nadiah Abu, Nurul Elyani Mohamed, Swee Keong Yeap, Kian Lam Lim, M. Nadeem Akhtar, Aimi Jamil Zulfadli, Beh Boon Kee, Mohd Puad Abdullah, Abdul Rahman Omar, Noorjahan Banu Alitheen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Flavokawain B (FKB) is a naturally occurring chalcone that can be isolated through the root extracts of the kava-kava plant (Piper methysticum). It can also be synthesized chemically to increase the yield. This compound is a promising candidate as a biological agent, as it is reported to be involved in a wide range of biological activities. Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro. However, the in vivo antitumor effects of FKB have not been reported on yet. Breast cancer is one of the major causes of cancer-related deaths in the world today. Any potential treatment should not only impede the growth of the tumor, but also modulate the immune system efficiently and inhibit the formation of secondary tumors. As presented in our study, FKB induced apoptosis in 4T1 tumors in vivo, as evidenced by the terminal deoxynucleotidyl transferase dUTP nick end labeling and hematoxylin and eosin staining of the tumor. FKB also regulated the immune system by increasing both helper and cytolytic T-cell and natural killer cell populations. In addition, FKB also enhanced the levels of interleukin 2 and interferon gamma but suppressed interleukin 1B. Apart from that, FKB was also found to inhibit metastasis, as evaluated by clonogenic assay, bone marrow smearing assay, real-time polymerase chain reaction, western blot, and proteome profiler analysis. All in all, FKB may serve as a promising anticancer agent, especially in treating breast cancer.

Original languageEnglish
Pages (from-to)1401-1417
Number of pages17
JournalDrug Design, Development and Therapy
Volume9
DOIs
Publication statusPublished - 6 Mar 2015
Externally publishedYes

Fingerprint

Breast Neoplasms
Kava
Neoplasms
Immune System
Chalcone
DNA Nucleotidylexotransferase
flavokawain B
Interleukins
Biological Factors
Proteome
Hematoxylin
Eosine Yellowish-(YS)
Helper-Inducer T-Lymphocytes
Natural Killer Cells
Antineoplastic Agents
Interferon-gamma
Interleukin-2
Real-Time Polymerase Chain Reaction
Bone Marrow
Western Blotting

Keywords

  • 4T1
  • Cancer
  • Flavokawain B
  • Kava-kava
  • Metastasis

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Abu, N., Mohamed, N. E., Yeap, S. K., Lim, K. L., Akhtar, M. N., Zulfadli, A. J., ... Alitheen, N. B. (2015). In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice. Drug Design, Development and Therapy, 9, 1401-1417. https://doi.org/10.2147/DDDT.S67976

In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice. / Abu, Nadiah; Mohamed, Nurul Elyani; Yeap, Swee Keong; Lim, Kian Lam; Akhtar, M. Nadeem; Zulfadli, Aimi Jamil; Kee, Beh Boon; Abdullah, Mohd Puad; Omar, Abdul Rahman; Alitheen, Noorjahan Banu.

In: Drug Design, Development and Therapy, Vol. 9, 06.03.2015, p. 1401-1417.

Research output: Contribution to journalArticle

Abu, N, Mohamed, NE, Yeap, SK, Lim, KL, Akhtar, MN, Zulfadli, AJ, Kee, BB, Abdullah, MP, Omar, AR & Alitheen, NB 2015, 'In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice', Drug Design, Development and Therapy, vol. 9, pp. 1401-1417. https://doi.org/10.2147/DDDT.S67976
Abu, Nadiah ; Mohamed, Nurul Elyani ; Yeap, Swee Keong ; Lim, Kian Lam ; Akhtar, M. Nadeem ; Zulfadli, Aimi Jamil ; Kee, Beh Boon ; Abdullah, Mohd Puad ; Omar, Abdul Rahman ; Alitheen, Noorjahan Banu. / In vivo antitumor and antimetastatic effects of flavokawain B in 4T1 breast cancer cell-challenged mice. In: Drug Design, Development and Therapy. 2015 ; Vol. 9. pp. 1401-1417.
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