In vivo antimalarial activity of the endophytic actinobacteria, Streptomyces SUK 10

Mohd Shukri Baba, Noraziah Mohamad Zin, Zainal Abidin Abu Hassan, Jalifah Latip, Florence Pethick, Iain S. Hunter, Ru Angelie Edrada-Ebel, Paul R. Herron

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Endophytic bacteria, such as Streptomyces, have the potential to act as a source for novel bioactive molecules with medicinal properties. The present study was aimed at assessing the antimalarial activity of crude extract isolated from various strains of actinobacteria living endophytically in some Malaysian medicinal plants. Using the four day suppression test method on male ICR strain mice, compounds produced from three strains of Streptomyces (SUK8, SUK10, and SUK27) were tested in vivo against Plasmodium berghei PZZ1/100 in an antimalarial screen using crude extracts at four different concentrations. One of these extracts, isolated from Streptomyces SUK10 obtained from the bark of Shorea ovalis tree, showed inhibition of the test organism and was further tested against P. berghei-infected mice for antimalarial activity at different concentrations. There was a positive relationship between the survival of the infected mouse group treated with 50 µg/kg body weight (bw) of ethyl acetate-SUK10 crude extract and the ability to inhibit the parasites growth. The parasite inhibition percentage for this group showed that 50% of the mice survived for more than 90 days after infection with the parasite. The nucleotide sequence and phylogenetic tree suggested that Streptomyces SUK10 may constitute a new species within the Streptomyces genus. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.

Original languageEnglish
Pages (from-to)847-855
Number of pages9
JournalJournal of Microbiology
Volume53
Issue number12
DOIs
Publication statusPublished - 1 Dec 2015

Fingerprint

Actinobacteria
Antimalarials
Streptomyces
Complex Mixtures
Plasmodium berghei
Parasites
Dipterocarpaceae
Inbred ICR Mouse
Parasitic Diseases
Drug Discovery
Medicinal Plants
Body Weight
Bacteria
Growth
Pharmaceutical Preparations

Keywords

  • antimalaria
  • endophytic
  • Streptomyces SUK10

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

In vivo antimalarial activity of the endophytic actinobacteria, Streptomyces SUK 10. / Baba, Mohd Shukri; Mohamad Zin, Noraziah; Hassan, Zainal Abidin Abu; Latip, Jalifah; Pethick, Florence; Hunter, Iain S.; Edrada-Ebel, Ru Angelie; Herron, Paul R.

In: Journal of Microbiology, Vol. 53, No. 12, 01.12.2015, p. 847-855.

Research output: Contribution to journalArticle

Baba, MS, Mohamad Zin, N, Hassan, ZAA, Latip, J, Pethick, F, Hunter, IS, Edrada-Ebel, RA & Herron, PR 2015, ' In vivo antimalarial activity of the endophytic actinobacteria, Streptomyces SUK 10', Journal of Microbiology, vol. 53, no. 12, pp. 847-855. https://doi.org/10.1007/s12275-015-5076-6
Baba, Mohd Shukri ; Mohamad Zin, Noraziah ; Hassan, Zainal Abidin Abu ; Latip, Jalifah ; Pethick, Florence ; Hunter, Iain S. ; Edrada-Ebel, Ru Angelie ; Herron, Paul R. / In vivo antimalarial activity of the endophytic actinobacteria, Streptomyces SUK 10. In: Journal of Microbiology. 2015 ; Vol. 53, No. 12. pp. 847-855.
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