In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice

Nadiah Abu, Nurul Elyani Mohamed, Nirosha Tangarajoo, Swee Keong Yeap, M. Nadeem Akhtar, Mohd Puad Abdullah, Abdul Rahman Omar, Noorjahan Banu Alitheen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Flavokawains are chalcones that can be found in the root extracts of the kava-kava (Piper methysticum) plant. Flavokawain A and flavokawain B are known to possess potential anti-inflammation and anti-cancer activities. Nevertheless, the effects of both these compounds on the normal function of the host have not been studied. There is a need to find agents that can enhance the functionality of the immune system without disturbing the homeostatic balance. This study aimed to determine the toxicity and immunomodulatory effects of flavokawain A and flavokawain B on Balb/c mice. Several assays were conducted, the MTT viability assay, cytokine detection (IL-2 and TNF-α), immunophenotyping of important immune markers, serum biochemical analysis and detection of nitric oxide levels. Based on our results, flavokawain A and B did not cause mortality and all mice were observed normal after the treatment period. Both flavokawains stimulated splenocyte proliferation, the secretion of IL-2 and TNF-α and raised the population of T cell subsets without significantly altering the level of several serum biochemical parameters. Overall, flavokawain A and B could serve as potential immune-modulator drugs without causing any toxicity, however further in vivo evidence is needed.

Original languageEnglish
Pages (from-to)1199-1202
Number of pages4
JournalNatural Product Communications
Volume10
Issue number7
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

kava
interleukin-2
Piper methysticum
Kava
chalcones
toxicity
blood chemistry
mice
assays
splenocytes
Interleukin-2
nitric oxide
immune system
cytokines
T-lymphocytes
inflammation
viability
secretion
Chalcones
drugs

Keywords

  • Chalcone
  • Flavokawain
  • Immunomodulatory
  • Toxicity

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Plant Science
  • Complementary and alternative medicine

Cite this

Abu, N., Mohamed, N. E., Tangarajoo, N., Yeap, S. K., Nadeem Akhtar, M., Abdullah, M. P., ... Alitheen, N. B. (2015). In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice. Natural Product Communications, 10(7), 1199-1202.

In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice. / Abu, Nadiah; Mohamed, Nurul Elyani; Tangarajoo, Nirosha; Yeap, Swee Keong; Nadeem Akhtar, M.; Abdullah, Mohd Puad; Omar, Abdul Rahman; Alitheen, Noorjahan Banu.

In: Natural Product Communications, Vol. 10, No. 7, 01.01.2015, p. 1199-1202.

Research output: Contribution to journalArticle

Abu, N, Mohamed, NE, Tangarajoo, N, Yeap, SK, Nadeem Akhtar, M, Abdullah, MP, Omar, AR & Alitheen, NB 2015, 'In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice', Natural Product Communications, vol. 10, no. 7, pp. 1199-1202.
Abu N, Mohamed NE, Tangarajoo N, Yeap SK, Nadeem Akhtar M, Abdullah MP et al. In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice. Natural Product Communications. 2015 Jan 1;10(7):1199-1202.
Abu, Nadiah ; Mohamed, Nurul Elyani ; Tangarajoo, Nirosha ; Yeap, Swee Keong ; Nadeem Akhtar, M. ; Abdullah, Mohd Puad ; Omar, Abdul Rahman ; Alitheen, Noorjahan Banu. / In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice. In: Natural Product Communications. 2015 ; Vol. 10, No. 7. pp. 1199-1202.
@article{6677fb4059724e0a90f1cd9e5b9eb72e,
title = "In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice",
abstract = "Flavokawains are chalcones that can be found in the root extracts of the kava-kava (Piper methysticum) plant. Flavokawain A and flavokawain B are known to possess potential anti-inflammation and anti-cancer activities. Nevertheless, the effects of both these compounds on the normal function of the host have not been studied. There is a need to find agents that can enhance the functionality of the immune system without disturbing the homeostatic balance. This study aimed to determine the toxicity and immunomodulatory effects of flavokawain A and flavokawain B on Balb/c mice. Several assays were conducted, the MTT viability assay, cytokine detection (IL-2 and TNF-α), immunophenotyping of important immune markers, serum biochemical analysis and detection of nitric oxide levels. Based on our results, flavokawain A and B did not cause mortality and all mice were observed normal after the treatment period. Both flavokawains stimulated splenocyte proliferation, the secretion of IL-2 and TNF-α and raised the population of T cell subsets without significantly altering the level of several serum biochemical parameters. Overall, flavokawain A and B could serve as potential immune-modulator drugs without causing any toxicity, however further in vivo evidence is needed.",
keywords = "Chalcone, Flavokawain, Immunomodulatory, Toxicity",
author = "Nadiah Abu and Mohamed, {Nurul Elyani} and Nirosha Tangarajoo and Yeap, {Swee Keong} and {Nadeem Akhtar}, M. and Abdullah, {Mohd Puad} and Omar, {Abdul Rahman} and Alitheen, {Noorjahan Banu}",
year = "2015",
month = "1",
day = "1",
language = "English",
volume = "10",
pages = "1199--1202",
journal = "Natural Product Communications",
issn = "1934-578X",
publisher = "Natural Product Communications",
number = "7",

}

TY - JOUR

T1 - In vitro toxicity and in vivo immunomodulatory effects of flavokawain a and flavokawain B in balb/C mice

AU - Abu, Nadiah

AU - Mohamed, Nurul Elyani

AU - Tangarajoo, Nirosha

AU - Yeap, Swee Keong

AU - Nadeem Akhtar, M.

AU - Abdullah, Mohd Puad

AU - Omar, Abdul Rahman

AU - Alitheen, Noorjahan Banu

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Flavokawains are chalcones that can be found in the root extracts of the kava-kava (Piper methysticum) plant. Flavokawain A and flavokawain B are known to possess potential anti-inflammation and anti-cancer activities. Nevertheless, the effects of both these compounds on the normal function of the host have not been studied. There is a need to find agents that can enhance the functionality of the immune system without disturbing the homeostatic balance. This study aimed to determine the toxicity and immunomodulatory effects of flavokawain A and flavokawain B on Balb/c mice. Several assays were conducted, the MTT viability assay, cytokine detection (IL-2 and TNF-α), immunophenotyping of important immune markers, serum biochemical analysis and detection of nitric oxide levels. Based on our results, flavokawain A and B did not cause mortality and all mice were observed normal after the treatment period. Both flavokawains stimulated splenocyte proliferation, the secretion of IL-2 and TNF-α and raised the population of T cell subsets without significantly altering the level of several serum biochemical parameters. Overall, flavokawain A and B could serve as potential immune-modulator drugs without causing any toxicity, however further in vivo evidence is needed.

AB - Flavokawains are chalcones that can be found in the root extracts of the kava-kava (Piper methysticum) plant. Flavokawain A and flavokawain B are known to possess potential anti-inflammation and anti-cancer activities. Nevertheless, the effects of both these compounds on the normal function of the host have not been studied. There is a need to find agents that can enhance the functionality of the immune system without disturbing the homeostatic balance. This study aimed to determine the toxicity and immunomodulatory effects of flavokawain A and flavokawain B on Balb/c mice. Several assays were conducted, the MTT viability assay, cytokine detection (IL-2 and TNF-α), immunophenotyping of important immune markers, serum biochemical analysis and detection of nitric oxide levels. Based on our results, flavokawain A and B did not cause mortality and all mice were observed normal after the treatment period. Both flavokawains stimulated splenocyte proliferation, the secretion of IL-2 and TNF-α and raised the population of T cell subsets without significantly altering the level of several serum biochemical parameters. Overall, flavokawain A and B could serve as potential immune-modulator drugs without causing any toxicity, however further in vivo evidence is needed.

KW - Chalcone

KW - Flavokawain

KW - Immunomodulatory

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=84946904632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946904632&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 1199

EP - 1202

JO - Natural Product Communications

JF - Natural Product Communications

SN - 1934-578X

IS - 7

ER -