In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine

Noradilah Samseh Abdullah, Mohamed Kamel Abdul Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this study were polyhexamethylene biguanide (PHMB) and chlorhexidine and both were serially diluted. Cyst suspensions from all three strains were tested against the antimicrobial agents, respectively. After 48 h of incubation at 37°C, the suspension was filtered and the filter membrane was placed onto non-nutrient agar plate lawned with heat-killed Escherichia coli. The plates were examined daily under the inverted microscope until day 14 but were negative for Acanthamoeba trophozoites. The presence of trophozoites indicated ineffectiveness of the antimicrobial agents. Both antimicrobial agents used were found to be effective against Acanthamoeba cysts for all the strains tested. PHMB gave minimum cysticidal concentration (MCC) mean value of 4.232 μg/mL and chlorhexidine showed MCC mean value of 3.906 μg/mL. So, from this study, it can be concluded that PHMB and chlorhexidine were effective in killing the tested Acanthamoeba cysts.

Original languageEnglish
Pages (from-to)569-572
Number of pages4
JournalSains Malaysiana
Volume41
Issue number5
Publication statusPublished - May 2012

Fingerprint

Acanthamoeba
Chlorhexidine
Keratitis
Anti-Infective Agents
Cysts
Trophozoites
HS 6
Suspensions
Acanthamoeba Keratitis
Eye Infections
Vision Disorders
Agar
Hot Temperature
In Vitro Techniques
polyhexamethylene biguanide
Escherichia coli
Membranes

Keywords

  • Acanthamoeba
  • Chlorhexidine
  • In vitro
  • Polyhexamethylene biguanide
  • Sensitivity

ASJC Scopus subject areas

  • General

Cite this

In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine. / Abdullah, Noradilah Samseh; Abdul Ghani, Mohamed Kamel; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Ahmad, Norazah.

In: Sains Malaysiana, Vol. 41, No. 5, 05.2012, p. 569-572.

Research output: Contribution to journalArticle

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