In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected tropical medicinal herbs and food plants

Fawzi Mahomoodally, Osman Mesaik Mohammed Ahmed Hassan, Iqbal M. Choudhary, Anwar H. Subratty, Ameenah Gurib-Fakim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To evaluate . in vitro immunomodulating properties and potential cytotoxicity of six tropical medicinal herbs and food plants namely . Antidesma madagascariense (Euphorbiaceae) (AM), . Erythroxylum macrocarpum (Erythroxylaceae) (EM), . Faujasiopsis flexuosa (Asteraceae) (FF), . Pittosporum senacia (Pittosporaceae) (PS), . Momordica charantia (Cucurbitaceae) (MC) and . Ocimum tenuiflorum (Lamiaceae) (OT). Methods: Initially, the crude water and methanol extracts were probed for their capacity to trigger immune cells' NADPH oxidase and MPO-dependent activities as measured by lucigenin- and luminol-amplified chemiluminescence, respectively; as compared to receptor-dependent (serum opsonised zymosan- OPZ) or receptor-independent phorbol myristerate acetate (PMA). Results: Preliminary screening on whole human blood oxidative burst activity showed significant and concentration-dependent immunomodulating properties of three plants AM, FF and OT. Further investigations of the fractions on isolated human polymorphonuclear cells (PMNs) and mice monocytes using two different pathways for activation of phagocytic oxidative burst showed that ethyl acetate fraction was the most potent extract. None of the active samples had cell-death effects on human PMNs, under the assay conditions as determined by the trypan-blue exclusion assay. Since PMA and OPZ NADPH oxidase complex is activated . via different transduction pathways, these results suggest that AM, FF and OT does not affect a specific transductional pathway, but rather directly inhibit a final common biochemical target such as the NADPH oxidase enzyme and/or scavenges ROS. Conclusions: Our findings suggest that some of these plants extracts/fractions were able to modulate significantly immune response of phagocytes and monocytes at different steps, emphasizing their potential as a source of new natural alternative immunomodulatory agents.

Original languageEnglish
Pages (from-to)440-447
Number of pages8
JournalAsian Pacific Journal of Tropical Medicine
Volume5
Issue number6
DOIs
Publication statusPublished - Jun 2012
Externally publishedYes

Fingerprint

Edible Plants
Respiratory Burst
Rosales
NADPH Oxidase
Medicinal Plants
Cell Extracts
Reactive Oxygen Species
Erythroxylaceae
Monocytes
Acetates
Momordica charantia
Euphorbiaceae
Lamiaceae
Cucurbitaceae
Luminol
Asteraceae
Zymosan
Trypan Blue
Plant Extracts
Phagocytes

Keywords

  • Chemiluminescence
  • Immune cells
  • Immunomodulation
  • Oxidative burst
  • Traditional medicinal herbs

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected tropical medicinal herbs and food plants. / Mahomoodally, Fawzi; Mohammed Ahmed Hassan, Osman Mesaik; Choudhary, Iqbal M.; Subratty, Anwar H.; Gurib-Fakim, Ameenah.

In: Asian Pacific Journal of Tropical Medicine, Vol. 5, No. 6, 06.2012, p. 440-447.

Research output: Contribution to journalArticle

Mahomoodally, Fawzi ; Mohammed Ahmed Hassan, Osman Mesaik ; Choudhary, Iqbal M. ; Subratty, Anwar H. ; Gurib-Fakim, Ameenah. / In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected tropical medicinal herbs and food plants. In: Asian Pacific Journal of Tropical Medicine. 2012 ; Vol. 5, No. 6. pp. 440-447.
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