In vitro mineralisation of grafted ePTFE membranes carrying carboxylate groups

Norsyahidah Mohd Hidzir, David J.T. Hill, Darren Martin, Lisbeth Grøndahl

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In vitro mineralisation in simulated body fluid (SBF) of synthetic polymers continues to be an important area of research as the outcomes cannot be predicted. This study evaluates a series of ePTFE membranes grafted with carboxylate-containing copolymers, specifically using acrylic acid and itaconic acid for grafting. The samples differ with regards to graft density, carboxylate density and polymer topology. The type and amount of mineral produced in 1.5 × SBF was dependent on the sample characteristics as evident from XPS, SEM/EDX, and FTIR spectroscopy. It was found that the graft density affects the mineral phases that form and that low graft density appear to cause co-precipitation of calcium carbonate and calcium phosphate. Linear and branched graft copolymer topology led to hydroxyapatite mineralisation whereas crosslinked graft copolymers resulted in formation of a mixture of calcium-phosphate phases. This study demonstrates that in vitro mineralisation outcomes for carboxylate-containing graft copolymers are complex. The findings of this study have implications for the design of bioactive coatings and are important for understanding the bone-biomaterial interface.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalBioactive Materials
Volume2
Issue number1
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Graft copolymers
Grafts
Body fluids
Calcium phosphate
Membranes
Transplants
Minerals
Polymers
Topology
Acids
Calcium Carbonate
Calcium carbonate
Biocompatible Materials
Body Fluids
Durapatite
Coprecipitation
Hydroxyapatite
Biomaterials
Acrylics
Energy dispersive spectroscopy

Keywords

  • Carboxylate groups
  • Expanded poly(tetrafluoroethylene)
  • Graft copolymerisation
  • Simulated body fluid

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology
  • Biomaterials

Cite this

In vitro mineralisation of grafted ePTFE membranes carrying carboxylate groups. / Mohd Hidzir, Norsyahidah; Hill, David J.T.; Martin, Darren; Grøndahl, Lisbeth.

In: Bioactive Materials, Vol. 2, No. 1, 01.03.2017, p. 27-34.

Research output: Contribution to journalArticle

Mohd Hidzir, Norsyahidah ; Hill, David J.T. ; Martin, Darren ; Grøndahl, Lisbeth. / In vitro mineralisation of grafted ePTFE membranes carrying carboxylate groups. In: Bioactive Materials. 2017 ; Vol. 2, No. 1. pp. 27-34.
@article{58437dd5295b418a9d1812b4f865bcc7,
title = "In vitro mineralisation of grafted ePTFE membranes carrying carboxylate groups",
abstract = "In vitro mineralisation in simulated body fluid (SBF) of synthetic polymers continues to be an important area of research as the outcomes cannot be predicted. This study evaluates a series of ePTFE membranes grafted with carboxylate-containing copolymers, specifically using acrylic acid and itaconic acid for grafting. The samples differ with regards to graft density, carboxylate density and polymer topology. The type and amount of mineral produced in 1.5 × SBF was dependent on the sample characteristics as evident from XPS, SEM/EDX, and FTIR spectroscopy. It was found that the graft density affects the mineral phases that form and that low graft density appear to cause co-precipitation of calcium carbonate and calcium phosphate. Linear and branched graft copolymer topology led to hydroxyapatite mineralisation whereas crosslinked graft copolymers resulted in formation of a mixture of calcium-phosphate phases. This study demonstrates that in vitro mineralisation outcomes for carboxylate-containing graft copolymers are complex. The findings of this study have implications for the design of bioactive coatings and are important for understanding the bone-biomaterial interface.",
keywords = "Carboxylate groups, Expanded poly(tetrafluoroethylene), Graft copolymerisation, Simulated body fluid",
author = "{Mohd Hidzir}, Norsyahidah and Hill, {David J.T.} and Darren Martin and Lisbeth Gr{\o}ndahl",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.bioactmat.2017.02.002",
language = "English",
volume = "2",
pages = "27--34",
journal = "Bioactive Materials",
issn = "2452-199X",
publisher = "KeAi Communications Co",
number = "1",

}

TY - JOUR

T1 - In vitro mineralisation of grafted ePTFE membranes carrying carboxylate groups

AU - Mohd Hidzir, Norsyahidah

AU - Hill, David J.T.

AU - Martin, Darren

AU - Grøndahl, Lisbeth

PY - 2017/3/1

Y1 - 2017/3/1

N2 - In vitro mineralisation in simulated body fluid (SBF) of synthetic polymers continues to be an important area of research as the outcomes cannot be predicted. This study evaluates a series of ePTFE membranes grafted with carboxylate-containing copolymers, specifically using acrylic acid and itaconic acid for grafting. The samples differ with regards to graft density, carboxylate density and polymer topology. The type and amount of mineral produced in 1.5 × SBF was dependent on the sample characteristics as evident from XPS, SEM/EDX, and FTIR spectroscopy. It was found that the graft density affects the mineral phases that form and that low graft density appear to cause co-precipitation of calcium carbonate and calcium phosphate. Linear and branched graft copolymer topology led to hydroxyapatite mineralisation whereas crosslinked graft copolymers resulted in formation of a mixture of calcium-phosphate phases. This study demonstrates that in vitro mineralisation outcomes for carboxylate-containing graft copolymers are complex. The findings of this study have implications for the design of bioactive coatings and are important for understanding the bone-biomaterial interface.

AB - In vitro mineralisation in simulated body fluid (SBF) of synthetic polymers continues to be an important area of research as the outcomes cannot be predicted. This study evaluates a series of ePTFE membranes grafted with carboxylate-containing copolymers, specifically using acrylic acid and itaconic acid for grafting. The samples differ with regards to graft density, carboxylate density and polymer topology. The type and amount of mineral produced in 1.5 × SBF was dependent on the sample characteristics as evident from XPS, SEM/EDX, and FTIR spectroscopy. It was found that the graft density affects the mineral phases that form and that low graft density appear to cause co-precipitation of calcium carbonate and calcium phosphate. Linear and branched graft copolymer topology led to hydroxyapatite mineralisation whereas crosslinked graft copolymers resulted in formation of a mixture of calcium-phosphate phases. This study demonstrates that in vitro mineralisation outcomes for carboxylate-containing graft copolymers are complex. The findings of this study have implications for the design of bioactive coatings and are important for understanding the bone-biomaterial interface.

KW - Carboxylate groups

KW - Expanded poly(tetrafluoroethylene)

KW - Graft copolymerisation

KW - Simulated body fluid

UR - http://www.scopus.com/inward/record.url?scp=85020003779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020003779&partnerID=8YFLogxK

U2 - 10.1016/j.bioactmat.2017.02.002

DO - 10.1016/j.bioactmat.2017.02.002

M3 - Article

AN - SCOPUS:85020003779

VL - 2

SP - 27

EP - 34

JO - Bioactive Materials

JF - Bioactive Materials

SN - 2452-199X

IS - 1

ER -