In vitro inhibitory effects of moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes

Boonyadist Vongsak, Wandee Gritsanapan, Yuvadee Wongkrajang, Ibrahim Jantan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3-O-glucoside and kaempferol 3-Oglucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3-O-glucoside, crypto-chlorogenic acid, and kaempferol 3-Oglucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 μM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 μM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components.

Original languageEnglish
Pages (from-to)1559-1561
Number of pages3
JournalNatural Product Communications
Volume8
Issue number11
Publication statusPublished - 2013

Fingerprint

Moringa oleifera
Respiratory Burst
chemiluminescence
phagocytes
Phagocytes
Luminescence
leaf extracts
Chlorogenic Acid
kaempferol
chlorogenic acid
quercetin
Inhibitory Concentration 50
glucosides
inhibitory concentration 50
extracts
Ethanol
ethanol
anti-inflammatory agents
Luminol
aspirin

Keywords

  • Chemiluminescence
  • Chemotaxis
  • Crypto-chlorogenic acid
  • Kaempferol 3-O-glucoside
  • Moringa oleifera
  • Quercetin 3-O-glucoside

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Complementary and alternative medicine
  • Plant Science

Cite this

In vitro inhibitory effects of moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes. / Vongsak, Boonyadist; Gritsanapan, Wandee; Wongkrajang, Yuvadee; Jantan, Ibrahim.

In: Natural Product Communications, Vol. 8, No. 11, 2013, p. 1559-1561.

Research output: Contribution to journalArticle

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