In-vitro inhibitory effect of Cinnamomum zeylanicum and Eugenia caryophyllata Oils on Multispecies Anaerobic Oral Biofilm

Nuramirah Azizan, Shahida Mohd Said, Mohd Khairul Firdaus Mazlan, Kurudeven Tamil Chelvan, Rohazila Mohamad Hanafiah, Zamirah Zainal Abidin

Research output: Contribution to journalArticle

Abstract

The study aimed to determine the in-vitro inhibitory effect of Cinnamomum zeylanicum Blume (cinnamon) and Eugenia caryophyllata (clove) oils on combined Porphyromonas gingivalis and Fusobacterium nucleatum biofilm. Following a steam distillation extraction technique, oils from cinnamon bark and clove buds were tested for their antibacterial anti-adhesion and anti-biofilm activities on mixed biofilm containing P.gingivalis and F.nucleatum. The quantification of viable and adhered bacterial cells on 96-well plates using the crystal violet test was analysed and morphology of the bacteria in biofilm was observed under the scanning electron microscope (SEM). Our study demonstrated C.zeylanicum oil inhibited satisfactory anti-adhesion effect and significantly disrupted the biofilm formation at 1.25-5.0 mg/mL whereas E.caryophyllata oil suppressed the adhesion of bacteria and disrupted the formation of biofilm at 0.63-5.0 mg/mL. The SEM study showed prominent changes including ruptures and flatten bacterial wall in samples with E.caryophyllata oil while pitted and flatten cells were seen in C.zeylanicum samples compared to the smooth and distinguished cell wall seen in untreated samples or even more damaged cells in Ampicillin samples. We conclude that C.zeylanicum and E.caryophyllata oils showed significant in vitro anti-biofilm properties against mixed P.gingivalis and F.nucleatum biofilm and prominently altered the bacterial wall morphology. This finding may suggest potential therapeutic properties of these oils in controlling biofilm-related oral diseases.

Original languageEnglish
Pages (from-to)411-417
Number of pages7
JournalJournal of International Dental and Medical Research
Volume12
Issue number2
Publication statusPublished - 1 Jan 2019

Fingerprint

Cinnamomum zeylanicum
Syzygium
Biofilms
Oils
Mouth Diseases
Clove Oil
Electrons
Fusobacterium nucleatum
Bacteria
Gentian Violet
In Vitro Techniques
Porphyromonas gingivalis
Distillation
Steam
Ampicillin
Cell Wall
Rupture

Keywords

  • Biofilm
  • Cinnamon
  • Clove
  • Electron microscopy
  • Periodontitis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

In-vitro inhibitory effect of Cinnamomum zeylanicum and Eugenia caryophyllata Oils on Multispecies Anaerobic Oral Biofilm. / Azizan, Nuramirah; Mohd Said, Shahida; Firdaus Mazlan, Mohd Khairul; Chelvan, Kurudeven Tamil; Hanafiah, Rohazila Mohamad; Zainal Abidin, Zamirah.

In: Journal of International Dental and Medical Research, Vol. 12, No. 2, 01.01.2019, p. 411-417.

Research output: Contribution to journalArticle

Azizan, Nuramirah ; Mohd Said, Shahida ; Firdaus Mazlan, Mohd Khairul ; Chelvan, Kurudeven Tamil ; Hanafiah, Rohazila Mohamad ; Zainal Abidin, Zamirah. / In-vitro inhibitory effect of Cinnamomum zeylanicum and Eugenia caryophyllata Oils on Multispecies Anaerobic Oral Biofilm. In: Journal of International Dental and Medical Research. 2019 ; Vol. 12, No. 2. pp. 411-417.
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