In-vitro immunomodulatory and anti-cancerous activities of biotransformed products of Dianabol through Azadirachta indica and its molecular docking studies

Saifullah, Saifullah Khan, Azizuddin, Sobia A. Halim, Muhammad Kashif, Almas Jabeen, Muhmammad Asif, Osman Mesaik Mohammed Ahmed Hassan, Zaheer Ul-Haq, Ahsana Dar, Muhammad I. Choudhary

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Plant Biotransformation is one of the tools for structural modifications of the organic substrate of low, moderate or high biological value utilizing plant cultured cells, these modifications of organic structures may lead to biologically augmented products and which may be ultimately substantial in cure or improvement of various morbidities and diseases.Results: Azadirachta indica A. Juss. suspension culture was employed for the biotransformation of dianabol (1) for the first time, and two metabolites, 17β-hydroxy-17α-methyl-5α-androst-1-en-3-one (2), and 17β-hydroxy-17α-methyl-5α-androstan-3-one (3) were obtained.Conclusions: Most important aspect of this work was the evaluation of metabolite 2, which strongly and differentially suppressed [not affecting whole blood and human polymorphonuclear cells (PMN)] the phytohemagglutinin (PHA)-activated T-cell proliferation (IC50: 50: 16.89 ± 1.32) unlike metabolite 3 and compound 1. Compound 2 also exhibited anticancer activity against lung cancer cell line; NCI-H460, it moderately inhibited the growth of cancer cells (22.5 ± 4.15 μM). Furthermore, a good correlation between the predicted binding energies of the compounds acquired by the FlexX program and the experimental affinities were speculated upon interacting with IL-2 protein during molecular docking studies.

Original languageEnglish
Article number163
JournalChemistry Central Journal
Volume7
Issue number1
DOIs
Publication statusPublished - 7 Oct 2013
Externally publishedYes

Fingerprint

Methandrostenolone
Metabolites
Cells
T-cells
Cell proliferation
Phytohemagglutinins
Binding energy
Interleukin-2
Suspensions
Blood
Substrates
Proteins
Biotransformation

Keywords

  • Anti-cancer activity
  • Anti-inflammatory activity
  • Biotransformation
  • Molecular docking studies

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

In-vitro immunomodulatory and anti-cancerous activities of biotransformed products of Dianabol through Azadirachta indica and its molecular docking studies. / Saifullah; Khan, Saifullah; Azizuddin; Halim, Sobia A.; Kashif, Muhammad; Jabeen, Almas; Asif, Muhmammad; Mohammed Ahmed Hassan, Osman Mesaik; Ul-Haq, Zaheer; Dar, Ahsana; Choudhary, Muhammad I.

In: Chemistry Central Journal, Vol. 7, No. 1, 163, 07.10.2013.

Research output: Contribution to journalArticle

Saifullah ; Khan, Saifullah ; Azizuddin ; Halim, Sobia A. ; Kashif, Muhammad ; Jabeen, Almas ; Asif, Muhmammad ; Mohammed Ahmed Hassan, Osman Mesaik ; Ul-Haq, Zaheer ; Dar, Ahsana ; Choudhary, Muhammad I. / In-vitro immunomodulatory and anti-cancerous activities of biotransformed products of Dianabol through Azadirachta indica and its molecular docking studies. In: Chemistry Central Journal. 2013 ; Vol. 7, No. 1.
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