In vitro immunomodulating properties of selected Sudanese medicinal plants

W. S. Koko, Osman Mesaik Mohammed Ahmed Hassan, S. Yousaf, M. Galal, M. Iqbal Choudhary

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Ethanolic extracts of 23 medicinal plants, commonly used in Sudanese folk medicines against infectious diseases, were investigated for their immunomodulating activity using luminol/lucigenin-based chemiluminescence assay. Preliminary screenings on whole blood oxidative burst activity showed inhibitory activities of 14 plant extracts, while only one plant, Balanites aegyptiaca fruits exhibited a proinflammatory activity. Further investigation was conducted by monitoring their effects on oxidative burst of isolated polymorphonuclear cells (PMNs) and mononuclear cells (MNCs) by using two different phagocytosis activators (serum opsonizing zymosan-A and PMA). Results obtained showed that the fruits and barks of Acacia nilotica, and leaves and barks of Khaya senegalensis, possess average inhibitory effects in the range of 70.7, 67.1, 69.5 and 67.4% on both types of phagocytes (PMNs and MNCs), respectively, at a 6.25 μg/mL concentration. Moderate inhibitory activity (52.2%) was exerted by the aerial parts of Xanthium brasilicum, while the rest of the plants showed only a weak inhibitory activity. The inhibition of oxidative burst activity was found to be irreversible in most of the extracts, except for Peganum harmala, Tephrosia apollinea, Tinospora bakis, and Vernonia amygdalina. Interestingly, the fruits of Balanites aegyptiaca exhibited a moderate proinflammatory effect (37-40.4% increases in ROS level compared to the control) at 25-100 μg/mL concentration in the case of whole blood along with PMNs phagocyte activity. The Tinospora bakis extract showed proinflammatory response at a low concentration (6.25 μg/mL) during activation with PMA. None of these extracts affected PMNs viability (90-98%) upon 2 h incubation, except of the ethanolic extracts of Acacia nilotica fruits and Balanites aegyptiaca barks.

Original languageEnglish
Pages (from-to)26-34
Number of pages9
JournalJournal of Ethnopharmacology
Volume118
Issue number1
DOIs
Publication statusPublished - 19 Jun 2008
Externally publishedYes

Fingerprint

Balanites
Medicinal Plants
Respiratory Burst
Fruit
Tinospora
Acacia
Phagocytes
Peganum
Tephrosia
Xanthium
Vernonia
Meliaceae
Luminol
Zymosan
Plant Extracts
Traditional Medicine
Luminescence
Phagocytosis
Communicable Diseases
In Vitro Techniques

Keywords

  • Chemiluminescence
  • Immunomodulation
  • Oxidative burst
  • Sudanese medicinal plants

ASJC Scopus subject areas

  • Pharmacology

Cite this

Koko, W. S., Mohammed Ahmed Hassan, O. M., Yousaf, S., Galal, M., & Choudhary, M. I. (2008). In vitro immunomodulating properties of selected Sudanese medicinal plants. Journal of Ethnopharmacology, 118(1), 26-34. https://doi.org/10.1016/j.jep.2008.03.007

In vitro immunomodulating properties of selected Sudanese medicinal plants. / Koko, W. S.; Mohammed Ahmed Hassan, Osman Mesaik; Yousaf, S.; Galal, M.; Choudhary, M. Iqbal.

In: Journal of Ethnopharmacology, Vol. 118, No. 1, 19.06.2008, p. 26-34.

Research output: Contribution to journalArticle

Koko, WS, Mohammed Ahmed Hassan, OM, Yousaf, S, Galal, M & Choudhary, MI 2008, 'In vitro immunomodulating properties of selected Sudanese medicinal plants', Journal of Ethnopharmacology, vol. 118, no. 1, pp. 26-34. https://doi.org/10.1016/j.jep.2008.03.007
Koko WS, Mohammed Ahmed Hassan OM, Yousaf S, Galal M, Choudhary MI. In vitro immunomodulating properties of selected Sudanese medicinal plants. Journal of Ethnopharmacology. 2008 Jun 19;118(1):26-34. https://doi.org/10.1016/j.jep.2008.03.007
Koko, W. S. ; Mohammed Ahmed Hassan, Osman Mesaik ; Yousaf, S. ; Galal, M. ; Choudhary, M. Iqbal. / In vitro immunomodulating properties of selected Sudanese medicinal plants. In: Journal of Ethnopharmacology. 2008 ; Vol. 118, No. 1. pp. 26-34.
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