In vitro expression of erythropoietin by transfected human mesenchymal stromal cells

P. L. Mok, S. K. Cheong, Leong Chooi Fun, Ainoon Othman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Mesenchymal stromal cells (MSC) are pluripotent progenitor cells that can be found in human bone marrow (BM). These cells have low immunogenicity and could suppress alloreactive T-cell responses. In the current study, MSC were tested for their capacity to carry and deliver the erythropoietin (EPO) gene in vitro. Methods: Expanded BM MSC was transfected with EPO-encoded plasmid pMCV1.2 and EPO-encoded MIDGE (minimalistic immunologically defined gene expression) vector by electroporation. The expressed EPO was used to induce hematopoietic stem cells (HSC) into erythroid colonies. Results: The results showed that the MIDGE vector was more effective and stable than the plasmid (pMCV1.2) in delivering EPO gene into MSC. The supernatants containing EPO obtained from the transfected cell culture were able to induce the differentiation of HSC into erythroid colonies. Discussion: MSC hold promise as a cell factory for the production of biologic molecules, and MIDGE vector is more effective and stable than the plasmid in nucleofection involving the EPO gene.

Original languageEnglish
Pages (from-to)116-124
Number of pages9
JournalCytotherapy
Volume10
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Erythropoietin
Mesenchymal Stromal Cells
Plasmids
Hematopoietic Stem Cells
Gene Expression
Genes
Electroporation
In Vitro Techniques
Stem Cells
Cell Culture Techniques
Bone Marrow
T-Lymphocytes

Keywords

  • Erythropoietin
  • Lipofection
  • Mesenchymal stromal cells
  • Minimalistic immunologically defined gene expression (MIDGE)
  • Nucleofection
  • Plasmid

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

In vitro expression of erythropoietin by transfected human mesenchymal stromal cells. / Mok, P. L.; Cheong, S. K.; Chooi Fun, Leong; Othman, Ainoon.

In: Cytotherapy, Vol. 10, No. 2, 2008, p. 116-124.

Research output: Contribution to journalArticle

Mok, P. L. ; Cheong, S. K. ; Chooi Fun, Leong ; Othman, Ainoon. / In vitro expression of erythropoietin by transfected human mesenchymal stromal cells. In: Cytotherapy. 2008 ; Vol. 10, No. 2. pp. 116-124.
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